당뇨병 흰쥐에서 식이제한이 간과 근육의 항산화효소 활성도에 미치는 영향

Abstract

Background : Oxidative stress is higher in obese diabetic than in non-diabetic subjects. Dietary caloric restriction(CR) without malnutrition is effective on both the control of body weight and reducing oxidative stress in obese diabetic patients. This experiments were subjected to CR and ad libitum feeding to evaluate their effects on oxidative stress in the liver and skeletal muscle of experimental animals. Methods : The 20 weeks old rats were studied: Otsuka Long-Evans Tokushima Fatty (OLETF) rats, as an animal model of type 2 diabetes, and control Long-Evans Tokushima Otsuka (LETO) rats. The experimental animals were subjected to 30% CR and ad libitum feeding for 3 weeks. The antioxidant enzyme activities in liver and skeletal muscle were measured. Result : CR was effective in reducing body weight and blood glucose concentrations (all p<0.01) both in OLET rats and LETO rats. The TBARS concentration in liver was decreased significantly by CR in both OLETF and LETO rats. The activities of catalase and superoxide dismutase(SOD) were increased by CR in liver of LETO rats. However, the changes of antioxidant enzymes activities by CR in liver of OLETF rats were not significant. In skeletal muscle, CR did not produce any significant effect on the antioxidant enzyme activities in both OLETF and LETO rats. Conclusions : These results demonstrate that CR is more effective to reduce oxidative stress in non-diabetic than diabetic animals especially in liver.