The antiviral effect of proteins and peptides derived from potatoes
- Issued Date
- 2006
- Abstract
- Molecular genetic research has been identifying the genes that could considerably increase crop yields through transgenic-mediated resistant to viral, insect, fungal and bacterial pathogens. Resistant to potato virus Y (Ry) gene confers extreme resistance to all strains of Potato Virus Y (PVY). To identify the virus resistant potato, 12 verities of Korean potato cultivars were infected by PVYO with the help of syringe. Of which 10 varieties were showed similar results in in vitro and in vivo experiments. Virus infected potatoes showed symptoms like mosaic, yellowing, necrosis, mottle, vein clearing, vein bending etc. Golden valley potato was found resistance to PVYO infection. Enzyme-Linked Immunosorbent Assay (ELISA) result also showed that Golden valley and Winter valley were found resistant and highly susceptible to the PVYO virus respectively. RT-PCR and Quantitative-PCR result showed that Ry gene was overexpressed 2.75 times more in PVYO infected Golden valley than the non-infected ones. PVYO virus movement was also observed in Winter valley potato after the 7, 14 and 21 dpi. Virus was moved rapidly toward growing regions of the plant parts. Potato protein and peptides from virus resistant potato Golden valley and Gogu valley showed antiviral activity. Alternatively, the development of biotic pesticide work is going on.
- Alternative Title
- 감자로부터 유도된 단백질과 펩타이드의 항바이러스성 효과
- Alternative Author(s)
- Park, Jae kyun
- Affiliation
- 조선대학교 대학원
- Department
- 일반대학원 생물신소재학과
- Advisor
- 鄭賢淑
- Awarded Date
- 2006-02
- Table Of Contents
- LIST OF FIGURES = III
LIST OF TABLES = IV
ABBREVIATIONS = V
ABSTRACT = VI
I. INTRODUCTION = 1
II. MATERIALS AND METHODS = 4
II - 1. PLANT MATERIALS AND IN VITRO STEM PROPAGATION = 4
II - 2. VIRUS INFECTION = 4
II - 3. TOTAL PROTEIN ASSAY = 5
II - 4. ELISA TEST FOR DETECT PVYO INFECTED POTATO VARIETIES = 5
II - 5. RT-PCR FOR DETECT PVYO INFECTED POTATO VARIETIES = 8
II - 5 - 1. Total RNA extraction = 8
II - 5 - 2. cDNA synthesis = 8
II - 5 - 3. RT-PCR amplification = 9
II - 6. PROTEIN EXTRACTION FROM POTATO LEAVES = 11
II - 7. PEPTIDE EXTRACTION FROM POTATO TUBERS = 11
II - 7 - 1. Purification and characterization of G4 and G11 peptides = 11
II - 7 - 2. Analogue 3 (A3) peptide synthesis = 13
II - 8. IN VIVO TREATMENT OF PROTEINS (GOLDEN VALLEY AND GOGU VALLEY), PEPTIDES (G4, G11 AND A3) TO INFECTED WINTER VALLEY LEAVES = 15
II - 9. QUANTITATION (REAL-TIME) PCR = 16
III. RESULTS = 18
III - 1. SYMTOMS BETWEEN POTATO VARIETIES BY PVYO INFECTION = 18
III - 2. SCREENING OF PVYO RESISTANT POTATO FROM 12 POTATO VARIETIES BY ELISA = 18
III - 3. RT-PCR FOR VIRUS DETECTION IN POTATOES AND EXPRESSION OF CP AND RY GENES = 21
III - 3 - 1. Expression of CP gene = 21
III - 3 - 2. Expression of Ry gene = 21
III - 4. VIRUS MOVEMENT = 22
III - 5. THE EFFECT OF IN VIVO PROTEINS AND PEPTIDES TREATMENT TO PVYO = 22
III - 5 - 1. Golden valley and Gogu valley proteins = 22
III - 5 - 2. G4, G11 and A3 peptides = 27
III - 6. QUANTITATION (REAL-TIME) PCR = 30
IV. DISCUSSION = 31
V. 적요 = 35
VI. REFERENCES = 36
- Degree
- Master
- Publisher
- 조선대학교 대학원
- Citation
- 박재균. (2006). The antiviral effect of proteins and peptides derived from potatoes.
- Type
- Dissertation
- URI
- https://oak.chosun.ac.kr/handle/2020.oak/6082
http://chosun.dcollection.net/common/orgView/200000232744
- Appears in Collections:
- General Graduate School > 3. Theses(Master)
- Files in This Item:
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