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Radicicol에 의한 65 단백질의 핵내 이동 억제에 관한 연구 : p65단백질의 핵내 이동에 관한 연구

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Author(s)
김상규
Issued Date
2006
Abstract
In this study, radicicol, a macrocyclic antifungal antibiotics originally isolated from Monosporium bonorden, inhibits LPS-induced expression of iNOS gene in RAW 264.7 cells. Treatment of peritoneal macrophages and RAW 264.7 cells with radicicol inhibited LPS-stimulated nitric oxide production in a dose-related manner. Immunohisto-chemical staining of iNOS and RT-PCR analysis showed that the decrease of NO was due to the inhibition of iNOS gene expression. Immunostaining of p65, electrophoretic mobility sift assay, and reporter gene assay showed that radicicol inhibited NF-k/Rel nuclear translocation, DNA binding, and transcriptional activation, respectively. Collectively, the experiments indicate that radicicol inhibits iNOS gene expression by blocking NF-k/Rel nuclear translocation. Due to the critical role that NO release plays in mediating inflammatory responses, the inhibitory effects of radicicol on iNOS suggest that radicicol may represent a useful anti-inflammatory agent. In this study, radicicol, a macrocyclic antifungal antibiotics originally isolated from Monosporium bonorden, inhibits LPS-induced expression of iNOS gene in RAW 264.7 cells. Treatment of peritoneal macrophages and RAW 264.7 cells with radicicol inhibited LPS-stimulated nitric oxide production in a dose-related manner. Immunohisto-chemical staining of iNOS and RT-PCR analysis showed that the decrease of NO was due to the inhibition of iNOS gene expression. Immunostaining of p65, electrophoretic mobility sift assay, and reporter gene assay showed that radicicol inhibited NF-k/Rel nuclear translocation, DNA binding, and transcriptional activation, respectively. Collectively, the experiments indicate that radicicol inhibits iNOS gene expression by blocking NF-k/Rel nuclear translocation. Due to the critical role that NO release plays in mediating inflammatory responses, the inhibitory effects of radicicol on iNOS suggest that radicicol may represent a useful anti-inflammatory agent.
Alternative Title
Inhibition of p65 nuclear translocation by radicicol
Alternative Author(s)
Kim, Sang-Gyu
Affiliation
조선대학교 대학원
Department
일반대학원 의학과
Advisor
이석기
Awarded Date
2006-02
Table Of Contents
ABSTRCT
I. INTRODUCTION = 1
II. MATERIALS AND METHODS = 3
A. Materials = 3
B. Cell culture = 3
C. Nitrite quantitation = 3
D. Western immunoblot analysis = 4
E. RT-PCR 4 F. Transient transfection of RAW 264.7 cells = 5
G. Electrophoretic mobility shift assay = 5
H. Statistical analysis = 6
III. RESULTS = 7
A. Effect of radicicol on nitrite production in macrophages. = 7
B. Inhibition of NF-kB/Rel in response to radicicol in LPS-stimulated RAW 264.7 cells. = 13
C. Inhibition of NF-kB/Rel nuclear translocation by radicicol in LPS-stimulated RAW 264.7 cells. = 16
IV. DISCUSSION = 22
V. REFERENCES = 25
Degree
Doctor
Publisher
조선대학교 대학원
Citation
김상규. (2006). Radicicol에 의한 65 단백질의 핵내 이동 억제에 관한 연구 : p65단백질의 핵내 이동에 관한 연구.
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/6069
http://chosun.dcollection.net/common/orgView/200000232732
Appears in Collections:
General Graduate School > 4. Theses(Ph.D)
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