CHOSUN

Noxa와 Mcl-1의 상호작용이 Noxa에 의한 세포사의 조절

Metadata Downloads
Author(s)
박선영
Issued Date
2006
Abstract
The Bcl-2 family proteins play critical roles in regulation of apoptosis, and the interaction of pro- and anti-apoptotic member is important to determine the fate of cells. Noxa belong to a Bcl-2 homology 3 (BH3)-only member of pro-apoptotic Bcl-2 family member. It has been originally identified to be upregulated by p53 in response to DNA damage. To understand the mechanism by which Noxa-induced cell death is regulated, we screened the binding partner using the yeast two hybrid screening. We found that Mcl-1 binds to Noxa. The binding of Noxa to Mcl-1 was confirmed by immunoprecipitation with anti-Mcl-1 in HCT 116 cells. Since Mcl-1 is known to inhibit cell death through
interaction with anti-apoptotic Bcl-2 family members, we investigated whether Mcl-1 can inhibit the Noxa-induced cell death. As expected, Mcl-1 significantly inhibits the Noxa-induced cell death in HCT 116 cells. To find out the mechanism, we investigated degradation of Mcl-1 by Noxa. Mcl-1 degradation was inhibited by z-VAD-fmk, a pan-caspase inhibitor. The results suggest that Mcl-1 might be a necessary event in Noxa-induced cell death.
Alternative Title
Regulation of Noxa-mediated cell death interaction between Noxa and Mcl-1
Alternative Author(s)
Park, Sun-Young
Affiliation
조선대학교 대학원
Department
일반대학원 생물신소재학과
Advisor
김 태 형
Awarded Date
2006-02
Table Of Contents
Ⅰ. ABSTRACT = 1
Ⅱ. 서론 = 3
Ⅲ. 실험재료 및 방법 = 8
1. 시약 = 8
2. 세포배양조건 = 8
3. Competent cell 준비 = 8
4. Transformation = 9
5. Plasmid DNA 분리 = 9
6. Expression plasmid의 transfection = 10
7. 세포용해 = 10
8. 단백질 정량 = 10
9. SDS PAGE gel 전기영동 = 11
10. Western blotting = 11
11. Immunoprecipitation = 11
Ⅳ. 결과 = 13
1. Yeast two hybrid = 13
2. Noxa와 Mcl 1의 결합 = 16
3. Noxa에 의해 유도되는 apoptosis를 억제하는 Mcl 1 = 18
4. Mcl 1의 degradation = 21
5. Caspase에 의한 Mcl 1의 degradation = 24
Ⅴ. 고찰 = 26
Ⅵ. 적요 = 29
Ⅶ. REFFERENCES = 30
Degree
Master
Publisher
조선대학교 대학원
Citation
박선영. (2006). Noxa와 Mcl-1의 상호작용이 Noxa에 의한 세포사의 조절.
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/6061
http://chosun.dcollection.net/common/orgView/200000232720
Appears in Collections:
General Graduate School > 3. Theses(Master)
Authorize & License
  • AuthorizeOpen
  • Embargo2008-09-01
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.