HPLC를 이용한 라세미 N-벤질옥시카르보닐 α-아미노산과 그 에틸에스터 유도체의 광학분할

Abstract

The N-Benzyloxycarbonyl(CBZ) group is one of the most commonly used protecting groups for amino acids. The liquid chromatographic enantiomer separation of N-CBZ protected α－amino acids and α－amino acids ethyl esters on four polysaccharide-derived chiral stationary phases(CSPs), such as Chiralcel OD, Chiralpak AD, Chiralpak IA and Chiralpak IB was performed. Chiralpak IB showed performance superior to Chiralpak IA for resolution of N-CBZ-α-amino acids and their ethyl esters. In general, Chiralpak IA, Chiralpak IB were well seperated more than N-CBZ protected the corresponding ethyl esters. Especially, the coated Chiralcel OD, Chiralpak AD showed higher enantioselectivities than the covalently bonded Chiralpak IA, Chiralpak IB for enantiomer separation ofN-CBZ-α-amino acids and their ethyl esters.