HPLC를 이용한 경구용 당뇨병 치료제의 신속한 동시분석

Abstract

The rapid simultaneous analysis was described of oral anti-diabetic drugs such as Metformin, Rosiglitazone malate, Pioglitazone, Chlorpropamide, Tolbutamide, Repaglinide, Glipizide, Gliclazide, Glibenclamide, Nateglinide, Glimepiride and Gliquidone by HPLC. These drugs were gradiently analyzed on a reverse phase column C18 capcellpak(4.6 x 150 mm I.D) with mobile phases system of acetonitrile and 5mM Pic B6(1-hexansulfonate Na) containing 0.1% phosphoric acid at a flow rate of 1 ml/min and UV 210nm detection. The problems caused by proposed simultaneous analysis are 1) appearance of Metformin peak at a front of solvent peak due to its short retention time, 2) overlapping of peaks of Tolbutamide and Glipizide, 3) low separation of Glibenclimide and Nateglinide, respectively. In order to solve the problem Therefore, the optimal analysis method using some isocratic conditions was developed. This developed method is expected to be used for the rapid analysis of oral anti-diabetic drugs and monitoring the related illegal drugs.