5'-nitro-indirubinoxime을 처리한 구강 편평세포암종 KB 세포에서 유전자 발현의 변화

Abstract

A novel indirubin analog, 5’-nitro-indirubinoxime inhibits cell proliferation and induces apoptosis against various human cancer cells. In this study, the author performed the microarray analysis to identify genes differentially expressed in the KB oral squamous carcinoma cells after treated with 5’-nitro-indirubinoxime. Among the 10,800 genes analyzed, 1,701 genes (15.8%) showed statistically different expression level in the 5'-nitro-indirubinoxime treated cells with respect to untreated control cells. Among those, 263 genes (15.5%) were down-regulated and 220 genes (12.9%) were up-regulated more than 2-fold. Functionally related gene clusters include genes associated with signal transduction (18.1%), especially genes related with apoptosis (3.5%) and cell cycle regulation (5.8%). Our application of microarray analysis on 5’-nitro-indirubinoxime treated oral cancer cells allows the identification of candidate genes for providing novel insights into the indirubin mediated antitumor activity.