양막 부착포가 창상치유에 미치는 영향에 관한 연구

Abstract

The amniotic membrane is an avascular tissue that forms the innermost layer of the placenta. It is composed of five layers: an epithelial monolayer, an acellular basement membrane, a compact layer, a mesenchymal cell layer, and a spongy layer. Amniotic membrane was used as a wound dressing for burn patients and in a variety of surgical procedure to promote epithelialization. The membrane prevent tissue adhesion and was reported to promote epithelialization, reduce pain, and prevent infection. Therefore, in the present study, we evaluated wound treatments with the membrane. Treatments evaluated were: amniotic membrane patch(amniotic group), polyurethane film(Tegaderm ^(TM), 3M, USA: wet group), and nonadherent dressing(dry group). In this study, on days 3 and 5, rate of epithelialization of amniotic group was significantly greater than those of wounds of wet and dry group. It suggested that amniotic membrane contains several growth factor proteins that might benefit epithelialization after amniotic membrane patch dressing. On days 10, all wound achieved 100% histological epithelialization but wet group had more developed skin appendages than amniotic and dry group. Results indicate that use of amiotic membrane patch as an biologic dressing can increase epithelialization in early phase on intractable ulcer. But it may inhibit collagen fiber arrangement and developing of skin appendages in late phase. These amniotic inhibiting effects will be studied in the future.