생쥐 소장에서 배양된 Interstitial cells of cajal의 전기적 활동도에 대한 Carbachol의 조절

Metadata Downloads
Issued Date
Interstitial cells of cajal (ICC) are pacemaker cells that activate the periodic spontaneous inward currents (pacemaker currents) responsible for the production of slow waves in gastrointestinal smooth muscles. The effects of carbachol on the pacemaker currents in cultured ICC from murine small intestine were investigated by using whole-cell patch-clamp techniques at a holding potential of -70 mV (equilibrium potential of Cl^(-)) and at 30 ℃. Tetrodotoxin, nifedipine, tetraethylammonium, 4-aminopyridine, or glibenclamide did not change the frequency and amplitude of pacemaker currents. CsCl or LiCl instead of NaCl also produced pacemaker currents. However, divalent cations (Ni^(2+), Cd^(2+), Mn^(2+), and Co^(2+)) except Ba^(2+), nonselective cation channel blockers (gadolinium and flufenamic acid), external Ca^(2+)-free solution, and a reduction of external Na^(+) from normal to 1 mM inhibited pacemaker currents indicating that nonselective cation channels are involved in their generation. Carbachol depolarized the membrane potential in current clamp mode and produced tonic inward pacemaker currents. Muscarinic M_(2) and M_(3) receptor antagonist (methoctramine and 4-DAMP), intracellular GDPβs blocked these carbachol-induced responses. However, GTPγS produced tonic inward currents mimicked those of carbachol. Carbachol continued to produce tonic inward currents in external Ca^(2+)-free solution or in the presence of chelerythrine, a protein kinase C inhibitor. However, carbachol-induced tonic inward currents were blocked by cyclopiazonic acid, a Ca^(2+)-ATPase inhibitor in the endoplasmic reticulum or by an external 1 mM Na^(+) solution, or by flufenamic acid. These results demonstrate that carbachol may modulate intestinal motility by acting on ICC by activating nonselective cation channels via the release of intracellular Ca^(2+) induced by muscarinic M_(3) and M_(2) receptor stimulation.
Alternative Title
Carbachol modulates pacemaker currents through the activation of nonselective cation channels in cultured interstitial cells of cajal from murine small intestine
조선대학교 대학원
일반대학원 의학과
Awarded Date
Table Of Contents
목차 = 1
도목차 = 2
Ⅰ 서론 = 6
Ⅱ 실험방법 = 9
l. 세포분리 = 9
2. 세포막 전압 및 전류의 기록 = 10
3. 실험 용액과 약물 = 10
4. 실험결과의 통계적 처리 = 11
Ⅲ 실험결과 = 12
1. ICC에서 기록되는 전기적 활동도 = 12
2. 향도잡이 전류에 대한 이온통로 차단제들의 효과 = 12
3. 향도잡이 전류에 대한 양이온들 및 비선택성 양이온 통로 차단제의 효과 = 12
4. 세포밖 저농도의 Na^(+)과 Ca^(2+) 농도에 대한 효과 = 13
5. 서파와 향도잡이 전류에 대한 carbachol의 효과 = 14
6. Carbachol작용에 대한 무스카린성 수용체 차단제들의 효과 = 14
7. Carbachol 작용에 대한 G-단백질 매개여부 = 15
8. Carbachol 작용에 대한 세포밖 Ca^(2+) 제거 용액과 cyclopiazonic acid의 효과 = 15
9. Carbachol 작용에 대한 세포밖 1 1mM Na^(+) 용액과 flufenamic acid의 효과 = 16
10. Carbachol 작용에 대한 protein kinase C 차단제의 효과 = 16
Ⅳ. 고찰 = 31
Ⅴ. 요약 = 36
Ⅵ. 참고문헌 = 38
신무경. (2005). 생쥐 소장에서 배양된 Interstitial cells of cajal의 전기적 활동도에 대한 Carbachol의 조절.
Appears in Collections:
General Graduate School > 4. Theses(Ph.D)
Authorize & License
  • AuthorizeOpen
  • Embargo2009-12-10
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.