Ras 과발현 세포의 이동 기작에 관련된 미세구조적인 변화와 dynamin II의 기능분석

Abstract

Functional and fine structural analysis of the dynamin II during Ras overexpressed cell migration

Lee, Chul Woo Advisor : Prof. Kim, Su-Kwan, D.D.S., M.S.D., Ph.D. Department of Dentistry, Graduate School of Chosun University

Dynamin has been implicated in the formation of nascent vesicles in both the endocytic and secretory pathways. However, recent reports suggested that dynamin has been implicated in altering cell membrane shape during cell migration associated with cytoskeleton related proteins. Myosin II has been reported that its function was implicated in maintaining the cell morphology and cellular movement. To identify potential association with between dynamin II and myosin II, we carried out reciprocal immunoprecipitateion (IP). Dynamin II was more largely expressed with myosin II in NIH3T3 (ras, ras transformed cell) than that of NIH3T3. We also confirmed that interaction of these two proteins was observed in confocal microscopy analysis. Interestingly, interaction and localization of these proteins were changed by PDGF-stimulated NIH3T3 cells. Upon treatment with PDGF to induce the lamellarpodian and cell migration, dynamin II became markedly associated with myosin II. Grb2 (Growth factor receptor-bound protein 2), 24-kD an adaptor protein, is known as an essential element of the Ras signaling pathway. We further identified Grb2 associated dynamin II and myosin II in Ras transformed NIH3T3 cells. The present results suggested that dynamin II may involve the intermediate messenger in Ras signaling transduction pathway leading to membrane ruffling and cell migration

Key words: Dynamin II, NIH3T3, NIH3T3 (ras), Myosin, Migration