NFI-C 결손 생쥐의 상아모세포 분화과정에서 DSPP와 BSP mRNA의 발현

Abstract

Expression of DSPP and BSP mRNAs during odontoblast differentiation in the NFI-C (-/-) mice

Jung, Hyun-Ku, D.D.S., M.S.D. Advisor : Prof. Lee, Chang-Seop, D.D.S., M.S.D. Department of Dentistry, Graduate School of Chosun University

Nuclear factor I (NFI) exist in the odontoblast and osteoblast. NFI-C null mice demonstrated aberrant odontoblast differentiation, abnormal dentin formation, and molar lacking roots. This study was to examin phenotype of the aberrant odontoblast in NFI-C null mice and to evaluate the expression of DSPP and BSP mRNAs in NFI-C null mice with in-situ hybridization.

The results were as follows: 1.In the NFI-C (-/-) mice, the crown dentin of molar showed normally formation, but there is no root dentin. 2.In the NFI-C (-/-) mice, the labial dentin of mandibular incisors showed relatively a lot of dentin formation, but the lingual dentin defect. 3.In the NFI-C (-/-) mice, the odontoblast of mandibular incisor revealed abnormal shape and trapped in osteodentin-like mineralized tissue. 4.In the NFI-C (-/-) mice, the odontoblast in the crown dentin of molar showed strong expression of DSPP, the odontoblast in the root dentin of molar was not expression of DSPP. In the NFI-C (-/-) mice the odontoblast in the mandibular incisor showed weekly expression of DSPP. 5.In the wild mice, the odontoblast of mandibular incisor was not expression of BSP, but in the NFI-C (-/-) mice the odontoblast of mandibular incisor showed strong expression of BSP.

These results suggest that odontoblast in the NFI-C (-/-) mice change the phenotype into osteoblast.

Key words: NFI-C, odontoblast differentiation, DSPP, BSP