HSF1 활성화 기전의 조절에 있어 HSC70의 역할

Abstract

Sohn Hong Bum Advisor : Prof. Jung-Hoon Yoon, DDS, Ph.D. Department of Dentistry Graduate School of Chosun University

Heat shock factor 1 (HSF1) plays an essential role in mediating cellular response to diverse forms of physiological stresses. However, the molecular process of HSF1 modulated by its interacting protein was poorly understood. Here we identified Hsc70 as an interacting protein of HSF1 using Mass spectrometry. HSF1 exists in unstressed cells as a complexed form with Hsc70. Interestingly, Hsc70 was required for the regulation of HSF1 during heat stress and subsequent target gene expression in mammalian cells. Moreover, cells transfected with siRNAs targeted to Hsc70 showed greatly decreased activation of HSF1. The expression of HSF1 target gene was reduced by inhibition of Hsc70 and showed loss of cell protection ability by stress-induced apoptosis. These results indicate that Hsc70 is necessary and sufficient for the regulation of HSF1.