참나물 추출물이 흰쥐 간조직의 항산화계와 지질 대사에 미치는 영향

Abstract

참나물(Pimpinella brachycarpa)은 고유의 민속식물로서 지혈, 대하, 해열, 고혈압, 중풍, 신경통 등의 효과가 알려져 있으며, 최근에는 항산화 효과도 있는 것으로 보고되고 있다. 따라서 본 실험에서는 항산화 효과를 체계적으로 검토하기 위하여 in vitro에서 참나물을 에탄올로 추출한 후 hexane, chloroform, ethylacetate, butanol, H₂O로 분획하고, 각 획분에 대해서 Rancimat test, 아질산염 소거능, DPPH test를 실시하여 항산화력을 검토하였으며 , in vivo에서 에탄올 투여로 중독된 흰쥐에 참나물 추출물을 투여하여 간세포독성에 미치는 항산화 효과를 검토하기 위하여 참나물 에탄올 추출물을 6주간 투여한 후 체중증가와 식이효율, 간조직중 항산화 효소인 superoxide dismutase, xanthine oxidase, catalase, glutathione peroxidase등의 활성도 및 항산화 물질인 glutathione 및 lipid peroxide등을 측정하였다.|This study was performed to investigate the effect of ethanol extract of Pimpinella brachycarpa on antioxidant activities and lipid metabolism in the liver of rats. The results obtained were as follows : 1.In Pimpinella brachycarpa, maltose was major in free sugars, proline in amino acids, linoleic acid in fatty acids, oxalic acid in organic acids, calcium in minerals, K+ in cations and Cl- in anions, respectively. 2.On antioxidative activity of Pimpinella brachycarpa in vitro, n-hexane fraction of Pimpinella brachycarpa ethanol extract was the highest among fractions, and activity of nitrite scavenging was the highest at pH 1.2, and the peroxide value for linoleic acid was increased during the storage. 3.In scavenging effect of oxygen free radicals generated in the ethanol- induced hepatotoxicity in the liver of rats, ① The growth rate of the rats was decreased by ethanol administration, however, was gradually increased to a little lower level than the normal group by administering Pimpinella brachycarpa ethanol extracts. ② It was also observed that the activities of superoxide dismutase(SOD) increased by ethanol were markedly decreased in Pimpinella brachycarpa ethanol extracts administered group as compared with control group. The glutathione(GSH) contents were decreased by ethanol adminstration, however, increased after administering Pimpinella brachycarpa ethanol extracts. 4.In antihypercholesterolemic effect of Pimpinella brachycarpa ethanol extract on the levels of lipid in serum and the liver of rats fed high cholesterol diet, ① The growth rate of the hypercholesterolemic group(control group) was higher than the normal group, whereas the groups administered Pimpinella brachycarpa ethanol extract were decreased. The liver weight in control group were significantly higher than that of normal group, but the that of control group with Pimpinella brachycarpa ethanol extract administration was lower than control group. ② The activities of alanine aminotransferase(ALT), asparate amino- transferase(AST) and alkaline phosphatase(ALP) in serum were rather lowered in the Pimpinella brachycarpa ethanol extract administration group than in the cholesterol administered group. ③ Contents of total cholesterol and LDL-cholesterol of in serum were lower in Pimpinella brachycarpa ethanol extract administration groups than control group while those of HDL-cholesterol were higher. Pimpinella brachycarpa ethanol extract increased HDL-C/T-C ratio and lowered atherogenic index. Triglyceride content was not decreased significantly during 6 weeks of experiment, but the contents of free cholesterol and cholesteryl ester were significantly decreased in Pimpinella brachycarpa ethanol extract administrated groups than control group. The phospholipid content in serum was not affected significantly by Pimpinella brachycarpa ethanol extract, but it was gradually increased. The hepatic contents of total cholesterol and triglyceride were lowered in Pimpinella brachycarpa ethanol extract administrated groups than control group. These results suggest that ethanol extract of Pimpinella brachycarpa have a possible positive effect on the liver function in hepatotoxicity-induced rats by ethanol administration, and the fatty liver in hyperlipidemia-induced rats by high cholesterol administration.