안구적출에 따른 위둔덕의 칼슘결합단백질과 c-fos의 변화

Abstract

Cacium-binding proteins(CBPs) play an important role in the protection, differentiation and reorganization of the central nervous system. The effects of neonatal retinal deafferentation on a CBPs, calbindin D-28k, parvalbumin and calretinin, were examined immunohistochemically in the superficial layer of the rat superior colliculus . Also early gene familly c-fos was examined to evaluate the neuronal characteristics after monocular enucleation. On the experimental side of superior colliculus, the number of calbindin D-28k-immunoreactive(IR) cells was reduced(77.4% compared to control),but not fibers. The number of parvalbumin-IR neurons and fibers was also reduced on the experimental side(88.5% compared to control). In the other hand, the calretinin-IR cells were dramatically increased(642% compared to control), carletinin-IR fibers were markedly decresed on the experimental side. Appearance of c-fos-like immunoreactivity was represented much more in the ipsilateral superior colliculus than contralateral side. These results suggest that the changes of retinotectal projection may alter the expressional pattern of CBPs in different manners; relatively relatively stable in calbindin and parvalbumin expression and plastic in calretinin and expression.