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백영고 버섯의 공학적 기법을 이용한 액체배양에 관한 연구

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Author(s)
南亨根
Issued Date
2004
Abstract
The objective of the present study was to determine the optimal culture conditions and medium composition for production of mycelial and exo- polysacchride in liquid-state fermentation from Pleurotus nebrodensis Inzenga. Optimal culture conditions for mycelial growth and exo-polysaccharide production in flask culture were determined in this research. The optimal temperature and pH were 25℃, and 6.5, respectively. The optimal culture volume was 50 mL in a 300 mL flask and shaking speed was 150 rpm. The optimal inoculum age and volume percent were 7 days and 5%(w/v), respectively. Among the 10 carbon source tested, glucose was the best carbon source. The maximum mycelial growth and exo-polysaccharide production were achieved in a 5%(w/v) glucose. The best nitrogen sources were polypeptone and yeast extract, respectively. The optimal concentrations of polypeptone and yeast extract were 1.0% (w/v) and 0.8% (w/v), respectively. K₂HPO₄ (0.12%(w/v) and MgSO₄·7H₂O (0.12%(w/v) were the most effective inorganic salts for mycelial growth and exo-polysaccharide production. In summary, the optimal medium composition was glucose 5%, polypeptone 1.0%, yeast extract 0.8%, K₂HPO₄ 0.12% and MgSO₄·7H₂O 0.12% (w/v). Jar fermentations were carried out to optimize the culture conditions for mycelial growth and exo-polysaccharide production. The optimal agitation speed and aeration rate were 150 rpm and 1.5 v.v.m., respectively. Under optimal culture conditions, the maximum mycelial growth and exo-polysaccharide production after 11 days with a 5 L jar fermenter containing the optimized medium were 12.84 g/L and 4.85 g/L, respectively. However, the fundamental information obtained this study is insufficient in the development of a efficient process for mycelial growth and exo-polysaccharide production from P. nebrodensis Inzenga. To meet the requirements of large-scale mycelial and exo-polysaccharide production, therefore, further studies are needed, including configuration of suitable reactor, optimization of culture conditions and characterization of exo-polysaccharide produced from P. nebrodensis Inzenga
Alternative Title
A Study on the Liquid Culture of Pleurotus nebrodensis Inzenga Using Engineering Technique
Alternative Author(s)
Nam, Hyung-Gun
Affiliation
朝鮮大學校 大學院
Department
일반대학원 화학공학과
Advisor
車月石
Awarded Date
2005-02
Table Of Contents
목차
List of Tables = ⅲ
List of Figures = ⅳ
ABSTRACT = ⅵ
Ⅰ. 서론 = 1
Ⅱ. 문헌연구 = 4
Ⅱ-1. 느타리속(Pleurotus spp)버섯의 생산 = 4
Ⅱ-1-1. 고체배양 = 4
Ⅱ-1-2. 액체배양 = 5
Ⅱ-2. Pleurotus spp의 약리적 특징 = 7
Ⅱ-3. 균사체 생산 = 9
Ⅱ-3-1. 산소공급조건 = 9
Ⅱ-3-2. Pellet형과 filamentous형 균사체 비증식 속도 = 10
Ⅱ-4. 항종양성 다당류 = 13
Ⅲ. 재료 및 방법 = 19
Ⅲ-1. 균주 및 보존 = 19
Ⅲ-2. 접종원 = 19
Ⅲ-3-1 Flask 배양 조건 = 20
Ⅲ-3-2 영양요구성 = 20
Ⅲ-4 Jar-fermentation = 22
Ⅲ-5 분석방법 = 23
Ⅳ. 결과 및 고찰 = 25
Ⅳ-1. 온도의 영향 = 25
Ⅳ-2. 접종원의 배양일수 및 접종량의 영향 = 27
Ⅳ-3. 초기의 pH의 영향 = 31
Ⅳ-4. Surface aeration의 영향 = 33
Ⅳ-5. Shaking speed의 영향 = 35
Ⅳ-6. 영양요구성 = 37
Ⅳ-6-1. 탄소원의 선발 및 최적 농도 = 37
Ⅳ-6-2. 질소원 선발 및 최적농도 = 41
Ⅳ-6-3. 무기염류 선발 및 최적농도 = 46
Ⅳ-7. Jar fermentation = 51
Ⅳ-7-1. Agitation speed의 영향 = 51
Ⅳ-7-2 Aeration의 영향 = 55
Ⅳ-7-3 최적 배양조건하에서의 time profile = 58
Ⅴ. 결론 = 60
참고문헌 = 62
Degree
Master
Publisher
朝鮮大學校 大學院
Citation
南亨根. (2004). 백영고 버섯의 공학적 기법을 이용한 액체배양에 관한 연구.
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/5657
Appears in Collections:
General Graduate School > 3. Theses(Master)
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