생쥐 작은창자의 Calcitonin gene-related peptide (CGRP)의 분포에 대한 연구

Abstract

The distribution of calcitonin gene-related peptide-like immunoreactivity(-LI) in the murine small bowel were studied by using immunohistochemistry, also. analized functionally by using electrophysiological method(patch, clamp). Immunohistochemical studies demonstrated that CGRP-LI is localized in both nerve fibers and myenteric ganglion cells in the whole-mount preparation of murine small intestine. Double labelling with CGRP and c-kit investigated by confocal scanning microscope was shown that CGRP-LI enteric nerve fiber surrounded the c-kit positive interstitial cells of Cajal(ICC). Electrophysiological finding revealed that treatment of CGRP inhibited electrical activity on culture ICC. Our results suggest a CGRP innervation of murine small bowel ICC. The released CGRP from enteric nerve terminal may induce relaxation of small bowel through the inhibition of ICC