상아질 및 백악질 관련 세포주에서 OD314의 분포에 관한 연구
- Issued Date
- There was lots of works to identify the cellular factors (specific gene or master gene) and their functions related with tooth development and differentiation, but the mechanism and function for the controls of each factors in cellular environment were still unclear. Recently OD314 has been identified as a odontoblast specific gene that might be have a function such as regeneration and differentiation, however this also has not been clue for its function in cells. In previous report we have described that OD314 may play a important role in mineralization process of odontoblast and also regulate odontoblast-related genes such as OC, ON, and DSPP. However, we need some broad speculation for this function to draw out exact cellular functions.
Therefore, using various cell lines related with odontoblast, cementoblast, and preosteoblast (NIH3T3, MDPC23, OD11, OCCM30, MC3T3-E1) we have conducted immunofluoresence microscopical analysis using OD314 antibody with DAPI and confirmed the protein expression of OD314 in nucleus using western blotting.
From the results we suggest that OD314 is a potential factor as described below hypothesis.
1) Outgrowth and differentiation related protein?
2) Migration related protein?
3) Trafficking related protein?
4) Forcal contact related protein?
5) Nucleus membrane protein?
- Alternative Title
- TheStudy on cellular distributio of the OD314 in cell lines related with dentin and cementum
- 조선대학교 대학원
- 일반대학원 치의학과
- Awarded Date
- Table Of Contents
- 목차 = i
ABSTRACT = iv
Ⅰ. 서론 = 1
Ⅱ. 실험재료 및 방법 = 3
1. 세포주와 배양 = 3
2. 항체의 제작 및 면역형광염색 = 4
3. Western Blotting 분석 = 6
Ⅲ. 실험결과 = 8
1. NIH3T3 (Mouse fibroblast-like cell) 세포주에서 OD3l4발현 = 8
2. MDPC23 (Mouse dental pupal cell 23) 세포주에서 OD314발현 = 9
3. OD-11 (Odontoblast-like cell) 세포주에서 OD314발현 = 10
4. OCCM30 (Cementoblast-like cell) 세포주에서 OD314발현 = 11
5. MC3T3-E1 (Preosteoblast-like cell) 세포주에서 OD314발현 = 12
6. 각 세포주에서 발현되는 OD314의 동일 패턴과 특이점 = 13
Ⅳ. 총괄 및 고안 = 15
Ⅴ. 결론 = 18
참고문헌 = 19
사진부도 설명 = 24
- 박민주. (2004). 상아질 및 백악질 관련 세포주에서 OD314의 분포에 관한 연구.
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