산화 스트레스에 의한 배양 망막색소상피세포의 혈관형성 세포활성인자의 발현

Abstract

Purpose : This study was undertaken to investigate whether expression of angiogenic cytokines is induced in the cultured RPE by oxidative stress. Methods : ARPE-19 cells were exposed to H_(2)O_(2) and paraquat for 24, 48, and 72 hours and assayed for the detection of IL-8 and monocyte chemotactic protein(MCP)-1 expression by reverse transcriptase polymerase chain reaction(RT-PCR) method. To see that antioxidant overcome the oxidative stress, we observed expression of IL-8 and MCP-1 gene after ARPE cells were treated by paraquat and antioxidants including N -acetylcysteine(NAC) and pyrrolidine dithiocarbamate(PDTC) at the same time for 72hr. Results : Expressions of angiogenic cytokines, IL-8 and MCP-1 increased by incubating with paraquat for 72hr. Antioxidants including NAC and PDTC decreased the expression of IL-8 and MCP-1 gene by counteracting the paraquat effect. Conclusion : Oxidative stress stimulates angiogenic cytokines including IL-8 and MCP-1 in the cultured RPE. These results may be significant on the understanding the cause of age-related macular degeneration and its treatment.