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산화 스트레스에 의한 배양 망막색소상피세포의 혈관형성 세포활성인자의 발현

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Author(s)
김근오
Issued Date
2004
Abstract
Purpose : This study was undertaken to investigate whether expression of angiogenic cytokines is induced in the cultured RPE by oxidative stress.
Methods : ARPE-19 cells were exposed to H_(2)O_(2) and paraquat for 24, 48, and 72 hours and assayed for the detection of IL-8 and monocyte chemotactic protein(MCP)-1 expression by reverse transcriptase polymerase chain reaction(RT-PCR) method. To see that antioxidant overcome the oxidative stress, we observed expression of IL-8 and MCP-1 gene after ARPE cells were treated by paraquat and antioxidants including N -acetylcysteine(NAC) and pyrrolidine dithiocarbamate(PDTC) at the same time for 72hr.
Results : Expressions of angiogenic cytokines, IL-8 and MCP-1 increased by incubating with paraquat for 72hr. Antioxidants including NAC and PDTC decreased the expression of IL-8 and MCP-1 gene by counteracting the paraquat effect.
Conclusion : Oxidative stress stimulates angiogenic cytokines including IL-8 and MCP-1 in the cultured RPE. These results may be significant on the understanding the cause of age-related macular degeneration and its treatment.
Alternative Title
Induction of angiogenic cytokines including IL-8 and MCP-1 on cultured RPE by oxidative stress
Affiliation
조선대학교 대학원
Department
일반대학원 의학과
Awarded Date
2004-08
Table Of Contents
목차 = i
표목차 = ii
도목차 = iii
ABSTRACT = 1
서론 = 2
연구 대상 및 방법 = 3
결과 = 6
고찰 = 8
참고문헌 = 10
Degree
Master
Publisher
조선대학교
Citation
김근오. (2004). 산화 스트레스에 의한 배양 망막색소상피세포의 혈관형성 세포활성인자의 발현.
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/5520
http://chosun.dcollection.net/common/orgView/200000231746
Appears in Collections:
General Graduate School > 3. Theses(Master)
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