망막색소상피세포에서 산화성 스트레스에 의한 MMP-2 분비의 촉진

Abstract

Purpose: To evaluate mechanism macular neovascularization in age-related macular degeneration (AMD), we examinated whether or not human retinal pigment epithelial cells, ARPE-19, secret angiogenic factors including Matrix Metalloproteinase-2 (MMP-2) and Matrix Metalloproteinase-9 (MMP-9) under oxidative stress induced by paraquat(PQ) Methods: ARPE-19 cells were exposed to PQ for 72 hours and then assayed for mRNA expression of MMP-2 and MMP-9 by reverse transcriptase polymerase chain reaction (RT-PCR) method. The culture free medium was assayed for protein production of MMP-2 and MMP-9 by Gelatin zymography. And the effect of antioxidant, N-acetyl cysteine (NAC) on the oxidative stressed ARPE-19 cells was measured. Results: MMP-2 secretion was increased in the oxidative stress group compared with the control but the increase of secretion was blocked by mixing NAC with PQ. Conclusion: Oxidative stress stimulates MMP-2 secretion in the cultured human retinal pigment epithelial cells, which could be helpful to understand the mechanism of neovascularization in AMD.