열처리된 무 추출물의 궤양성 대장염 예방 및 항염증 효과에 미치는 영향

Abstract

This study aimed to investigate the comparative evaluation of pharmacological efficacy between sulfasalazine alone and its combination with herbal medicine on dextran sodium sulfate (DSS)-induced UC in mice. Balb/c mice received 5% DSS in drinking water for 7 days to induce colitis. Animals were divided into five groups (n = 9): I-normal group, II-DSS control group, III-DSS + sulfasalazine (30 mg/kg) group, IV-DSS + sulfasalazine (60 mg/kg) group, and V-DSS + sulfasalazine (30 mg/kg) + radish extract mixture (30 mg /kg) (SRE) group. DSS-treated mice developed symptoms similar to those of human UC, such as severe bloody diarrhea and weight loss. SRE supplementation, as well as sulfasalazine, suppressed colonic length, and mucosal inflammatory infiltration. In addition, SRE treatment significantly reduced the expression of pro-inflammatory signaling molecules through the suppression of both MAPK and nuclear factor-kappa B (NF- B) signaling pathways and prevented the apoptosis of colon. Moreover, SRE administration significantly led to the up-regulation of anti-oxidant enzyme including SOD and Catalase. This is the first report that radish extract mixture combined with sulfasalazine protects one against experimental UC via the inhibition of both inflammation and apoptosis, which is highly similar to the standard-of-care sulfasalazin.