금은화의 폴리페놀, 플라보노이드, Chlorogenic acid의 최적 추출조건

Abstract

This study was carried out to compare of extraction efficiency for Lonicera Flos by extraction conditions. To compare the extraction efficiency of active ingredients by the extraction methods and ratio of water to ethanol (0, 20, 40, 60, 80 and 100% water) for Lonicera japonica Flos was extracted by ultrasonic extraction, shaking extraction water-bath extraction, and soaking extraction. Contents of phenolic compounds were the highest with 127.9 mg GAE/g DW in 60% ethanol by hot-water extraction method at 80℃ and contents of flavonoids were highest with 29.8 mg GAE/g DW in 80% ethanol by hot-water extraction method at 80℃. Contents of chlologenic acid were the highest with 31.8 mg/g in 80% ethanol by hot-water extraction method at 80℃. Extraction of the bioactive ingredient of Lonicera japonica Flos was effective in 60-80% ethanol by hot-water extraction.