Antioxidant and Antimicrobial Activity of Boswellia Serrata Extract for Cosmetic Ingredient
- Issued Date
- 2023
- Abstract
- Boswellia serrata resin, which is an important source of gum oleoresin known as Indian frankincense and is well documented for its pharmaceutical properties due to its chemical structure, antibacterial and antioxidant properties, and the presence of several compounds such as polyphenols, phenols, and terpenoids.
In the experimental study, it was observed that Boswellia Serrata resin extracts inhibited antibacterial activity in all strains for the relevant concentrations. The diameter of the zone of inhibition for the B. serrata extract for S. epidermidis ranged from 13.3±0.58 to 10.3±0.58 mm, for M. furfur from10.6±0.58 to 9±0 mm, for M. pachydermatis from13.25±0.35 to 9.75±1.06 mm, and for C. acnes from 11.83±0.29 to 9.5±0 mm in the anaerobic jar.
antioxidant activity was measured with DPPH It was confirmed that there was higher activity in the Water Fr. compared to the Other fractions; respectively, Water Fr. showed the highest scavenging activity 902.19 ± 35.53 µg/mL, Ethyl acetate Fr. 20436 ± 652.19 µg/mL ,70% EtOH Fr. 8627.74± 369.22 µg/mL, and the Hexane Fr. Shows No scavenging activity.
According to ABTS assay, the Water fraction demonstrated the maximum scavenging activity with a scavenging activity of 1845.08 ± 2265.74, followed by the ethyl acetate fraction with a scavenging activity of 12167.16 ± 8152.82 µg/mL.
polyphenol and flavonoid content in the Water Fr. was the highest; it reached TPC 32.15 ± 0.75 ⅿg/mL and TFC 20.29 ± 1.47 ⅿg/mL.
This study shows that the Boswellia serrata resin have some biological activities, and if they differ in terms of effectiveness and activity, they can be considered a good component of cosmetic products.
- Alternative Title
- 보스웰리아 (Boswellia serrata) 추출물의 향장소재 적용을 위한 항산화와 항균활성
- Alternative Author(s)
- Bssmah Ghazi Alraddadi
- Affiliation
- 조선대학교 산업기술창업대학원
- Department
- 산업기술창업대학원 미용향장학과
- Advisor
- 신현재
- Awarded Date
- 2023-08
- Table Of Contents
- Ⅰ. Introduction 1
1.1. Background of the study 1
1.1.1. Herbal cosmetics 1
1.1.2. Boswellia Serrata 3
1.1.3. Scientific classification 5
1.1.4. Economic importance 12
1.2. Antioxidant 18
1.2.1. Definition 18
1.2.2. Free Radicals and Oxidative Stress 19
1.2.3. Skin antioxidant 19
1.3. Antimicrobial. 22
1.3.1. Defense systems of the skin 22
1.3.2. Bacterial growth on skin 23
1.3.3. Protection by colonizing bacteria 23
1.3.4. Microbes that cause skin disease 24
1.3.5. Antibacterial 27
1.4. Research Trends and Composition 28
1.4.1. Research Trends 28
1.4.2. Structure of the study 30
Ⅱ. Literally review 31
2.1. Review of research on Boswellia serrata 31
2.1.1. Ingredients and compounds of Boswellia serrata 31
2.1.2. Bioactive compounds in Boswellia serrata 36
2.1.3. Boswellia serrata in cosmetology 39
2.2. A review of research on Boswellia serrata 47
Ⅲ. Materials and Methods 50
3.1. Experimental Materials and Reagents 50
3.1.1. Materials 50
3.1.2. Reagents 50
3.2. Extraction and Isolation 51
3.2.1. Sample collection 51
3.2.2. Extraction 51
3.2.2.1 Soxhlet Extraction 51
3.2.2.2 Immersion Extraction 53
3.2.3. floor separation 55
3.3. Antibacterial activity 56
3.3.1. Antibacterial agar disc diffusion assay 56
3.4. Antioxidant activity 57
3.4.1. DPPH free radical scavenging assay 57
3.4.2. ABTS radical scavenging assay 59
3.5. Total polyphenol and flavonoid contents. 61
3.5.1. Determination of total polyphenol content 61
3.5.2. Determination of total flavonoid content. 62
3.6. Analysis of polyphenol componds. 63
3.6.1 High performance liquid chromatography (HPLC) analysis 63
3.6.2 HPLC-MS/MS Analysis Method. 64
Ⅳ. Results and Discussion 65
4.1. Yield of extract according to Immersion extraction method 65
4.2. Antibacterial activity using well diffusion method 68
4.3. Results of antioxidant activity 71
4.3.1. DPPH free radical scavenging 71
4.3.2. ABTS radical scavenging 73
4.4. Results of total polyphenol and flavonoid contents 75
4.4.1 Total polyphenol contents (TPC) 75
4.4.2. Total flavonoid contents (TFC) 76
4.5. Analysis results of polyphenol componds 78
4.5.1 HPLC Analysis Results 78
4.5.2 HPLC-MS/MS Analysis Results. 81
Ⅴ. Discussion 86
Ⅵ. Conclusion 89
Ⅶ. Reference 90
- Degree
- Master
- Publisher
- 조선대학교 산업기술창업대학원
- Citation
- 바스마. (2023). Antioxidant and Antimicrobial Activity of Boswellia Serrata Extract for Cosmetic Ingredient.
- Type
- Dissertation
- URI
- https://oak.chosun.ac.kr/handle/2020.oak/17756
http://chosun.dcollection.net/common/orgView/200000685000
- Appears in Collections:
- Engineering > 3. Theses(Master)
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