쥐의 치주염 모델에서의 P. gingivalis Lipopolysaccharide에 대한 염증성 인자들의 반응발현에 대한 연구

Abstract

I. Introduction The purpose of this study was to evaluate how the expression of specific cytokines related to gingival inflammation. IL-1β, IL-6, COX-2, PGE2 and MMP-9 was changed according to the concentration of LPS injected in a rat periodontitis model.

II. Materials and Methods Changes in the inflammatory factors of gingival tissue were evaluated in a periodontal disease induction model in which silk-ligature was wrapped around the maxillary second molar in rats. The experiment was conducted in a total of 4 groups, and 4 male rats were randomly assigned to each group; 1) Non-ligature group (Non-periodontitis model), 2) LPS group 1 (this groups were injected with 2 μg/mL pgLPS), 3) LPS 2 group(this groups were injected with 8 μg/mL pgLPS), 4) LPS 3 group(this groups were injected with 20 μg/mL pgLPS). pgLPS was injected into the gingiva around maxillary 2nd molar 3 times for 3 weeks. The ELISA kit was used to measure the expression changes of IL-1β, IL-6, MMP-9, COX-2, and PGE2 in gingival tissues collected from all control and experimental groups.

III. Results The expressed IL-1β concentrations in gingival tissue were 0.591 ± 0.073 pg/ml, 0.859 ± 0.088 pg/ml, 1.767 ± 0.359 pg/ml and 17.103 ± 2.708 pg/ml in the non-ligation group, LPS 1 group, LPS 2 group, and LPS 3 group, respectively. The expressed IL-6 concentrations were 1.466 ± 0.075 pg/ml, 1.756 ± 0.303 pg/ml, 2.595 ± 0.396 pg/ml and 6.486 ± 1.621 pg/ml in the non-ligation group, LPS 1 group, LPS 2 group, and LPS 3 group, respectively. The concentrations of expressed MMP-9 were 0.073 ± 0.009 ng/ml, 0.081 ± 0.004 ng/ml, 0.158 ± 0.017 ng/ml and 0.216 ± 0.063 ng/ml in the non-ligation group, LPS 1 group, LPS 2 group, and LPS 3 group, respectively. The expressed COX-2 concentrations were 0.133 ± 0.003 ng/ml, 0.111 ± 0.010 ng/ml, 0.174 ± 0.024 ng/ml and 0.446 ± 0.100 ng/ml in the non-ligation group, LPS 1 group, LPS 2 group, and LPS 3 group, respectively. The expressed PGE2 concentrations were 0.082 ± 0.003 ng/ml, 0.079 ± 0.015 ng/ml, 0.085 ± 0.013 ng/ml and 4.736 ± 0.013 ng/ml in the non-ligation group, LPS 1 group, LPS 2 group, and LPS 3 group, respectively.

IV. Conclusion As the concentration of LPS injection was increased, the expression levels of IL-1β, IL-6, MMP-9, COX-2, and PGE2 were increased. LPS can change the expression of inflammatory factors such as cytokines in the gingival tissue even with a small concentration of LPS, and it is thought that the expression of inflammatory factors is more clear when the concentration is increased.