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Reversed phase liquid chromatographic enantiomeric separation of α-amino acids as N-fluorenylmethoxycarbonyl derivatives using a covalently bonded type chiral stationary phase based on cellulose derivative under fluorescence detection

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Author(s)
Suraj Adhikari Wonjae Lee
Issued Date
2018
Keyword
Amino acid Chiral stationary phase Enantiomer separation Fluorenylmethoxycarbonyl derivative
Abstract
Reversed phase liquid chromatographic enantiomer separation of α-amino acids as N-fluorenylmethoxycarbonyl (FMOC) derivatives was performed using a covalently bonded type chiral column based on cellulose tris (3,5- dimethylphenylcarbamate) under fluorescence detection. The effect of the different strong and weak acid additives with 90% methanol/water (V/V) as mobile phases on the enantiomeric separation of N-FMOC α-amino acids was investigated. Amongst them used in this study, the strongest acid additive (HClO4) in the mobile phase showed the greatest enantioselectivity and resolution, while the weakest acid additive (CH3COOH) showed the worst. This analytical method using fluorescence detection affords high sensitivity as well as selectivity for determination of enantiomeric purity of amino acids as N-FMOC derivatives and, furthermore, is expected to broaden the application of enantiomer separation of these nalytes in chirotechnology.
Publisher
조선대학교 약학연구소
Citation
Suraj Adhikari. (2018). Reversed phase liquid chromatographic enantiomeric separation of α-amino acids as N-fluorenylmethoxycarbonyl derivatives using a covalently bonded type chiral stationary phase based on cellulose derivative under fluorescence detection, Journal of The Pharmaceutical Research | Vol.39, No.1 p.39~ p.44
Type
Laboratory article
ISSN
1738-1029
URI
https://oak.chosun.ac.kr/handle/2020.oak/17218
http://www.chosun.ac.kr/user/indexSub.do?codyMenuSeq=24428628&siteId=jpr&dum=dum&boardId=175153&page=1&command=view&boardSeq=310746
Appears in Collections:
2018 > Vol.39, No.1
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