폴리페놀이 함유된 스위트 체리 추출물의 항산화 및 항염 활성

Abstract

The fruit of sweet cherry is highly popular by Korean consumers for its precociousness and quality such as sweet, color, and sour taste. Besides palatability, there is huge needs for the nutritional and functional information about sweet cheery nowdays. In this study, sequential solvent fractions of hot water extract and 70% ethanol extract were prepared from domestic (Imsil region) and foreign (Chile and Poland) sweet cherries (Prunus avium L.) to investigate their antioxidant and anti-inflammatory activities. The yield of the hot water extract from Chile one was 29.28%, and the solvent fraction yields of 70% ethanol, hexane, ethyl acetate (EA), and water residue were 28.49%, 0.6%, 1.14%, and 84.37%, respectively. The yield hot water extract from Poland was 30.39%, and the solvent fraction yields for 70% ethanol, hexane, ethyl acetate (EA), and water residue were 36.01%, 0.64%, 2.68%, and 84.57%, respectively. The yield of hot water extract from Imsil region was 22.66%, and the solvent fraction yields for 70% ethanol, hexane, ethyl acetate (EA), and water residue were 21.46%, 0.87%, 1.58%, and 90.58%, respectively. Water extract of Chilean cherry is CW, 70% alcohol extract CE, hexane extract CEH, ethyl acetate extract CEE, residue is CEW. And the water extract of Polish cherry is PW, 70% alcohol extract PE, hexane extract PEH, ethyl acetate extract PEE, and the residue is PEW. And the water extract of Imsil cherry is IW, 70% alcohol extract IE, hexane extract IEH, ethyl acetate extract IEE, and the residue is IEW. The total polyphenols content of the fraction was high in CEE, PEE, and IEE, and the total flavonoid content was high in PEE and IEE. The total flavonoid content in CE was low. Analysis of flavonol glycosides using HPLC showed a high content in PW and PE, and flavonol glycosides were identified as a result of HPLC/MS in all of CW, CE, PW, PE, IW, and IE. ABTS radical scavenging showed high content in CEE (133.53±13.26), PEE (62.27±3.08), IEE (136.77±3.77), and DPPH radical scavenging showed high content in CE (701.62±28.68), PE (6084.10±39.65), IE (133.53±39.93) showed high antioxidant activity. To measure anti-inflammatory activity, cell viability was measured using RAW 264.7 cells for hot water extracts and 70% EtOH extracts from overseas (Chile, Poland) and domestic (Imsil). None of them showed any toxicity compared to the control group. After simultaneous administration of CW, CE, PW, PE, IW, and IE to RAW 264.7 cell treated with LPS, the concentration-dependent inhibitory ability of NO production was confirmed as a result of Nitric Oxide (NO) measurement. As a result of Western Blot measurement after administration of IE to LPS-treated RAW 264.7 cells, the expression of iNOS and COX-2 proteins was significantly suppressed, and the expression level of IκBα affecting NF-κB activity was reviewed. It was confirmed that the sample (IE) significantly increased IκBα, and is expected to decrease the amount of NF-κB expression. The research results suggest that the sweet cherry fruit from domestic (Imsil) has high potential in antioxidant and anti-inflammation activity, so it could be used as a valuable ingredient in functional foods and cosmetic products.