묵은지로부터 분리된 Pediococcus inopinatus의 특성 규명 및 안전성 평가

Abstract

In this study, Pediococcus inopinatus strains, which were dominant microorganism in mukeunji, were isolated and then analyzed their characteristics and safeties. Forty samples of mukeunji were collected from 32 regions in Korea. Storage temperatures and aging periods of product mukeunji samples were investigated and their chemical properties were determined. Forty samples of mukeunji were collected to isolate the dominant lactic acid bacteria (LAB). And then identified the LAB isolated base on morphological characteristics, gram-staining and determination of 16S rRNA gene sequence. As a result, 31 strains of P. inopinatus were isolated. The result of measuring the pH of the culture medium after culturing the isolated P. inopinatus and the type strain. It was confirmed that the pH was 4.14 ± 0.10. As a result of analyzing sugar metabolism using the API 50 CHL kit, it was confirmed that it can metabolize an average of 11 to 16 sugars. The effect of culture temperature on the growth of P. inopinatus strains during cultivation was confirmed. It was confirmed that it grows best at 30°C. As a result of safety testing, none of the P. inopinatus strains showed α, β type hemolysis, and no harmful enzyme activity. Also P. inopinatus strains showed low antibiotic sensitivity to 6 antibiotics. As a result of observing whether or not p. inopinatus produced biogenic amine, it was observed that it did not produce biogenic amine. Through this study, it can be proved that the P. inopinatus strain, which dominates the mukeunji, is safe.