탄닌이 2,4-dinitrochloroben zene(DNCB) 피부염 생쥐의 모발성장에 미치는 영향

Abstract

ABSTRACT

The effect of tannin on hair growth in 2,4-Dinitrochlorobenzene(DNCB)-dermatitis mice.

Kyoung-Hwa Jang Advisor : Eun-Mi Ryu, Ph.D Dept. of Beauty and Cosmetic Graduate School of Chosun University

Appearance is being perceived as competitive by not only women but also men, so hair and scalp are exposed to chemicals such as frequent dyeing and bleaching and permanent for enhancing appearance. Those reasons cause problematic scalp disease or dermatitis on the scalp and hair growth impediment. The purpose of this study was to investigate the effects of tannin, which has antimicrobial and immunological properties, on hair growth in contact dermatitis induced by 2,4-dinitrochlorobenzene(DNCB). Twenty male 5 week old balb/c mice adapted for one week at Samtako were examined for a total of 8 weeks. First, mice(DNCB-DM) were exposed to dermatitis, and tannin aqueous solution (0.2w/v in 200ml of tap water) was orally taken to dermatitis induced mice(DNCB-DM+T). The results were as follows: First, skin symptoms of mice in the DNCB-DM+T group treated for 3 weeks and 5 weeks showed dermatitis symptoms such as skin rash, papule, eczema and scab formation, but significantly reduced over the DNCB-DM group. There was no change in skin symptoms in the control group, O,A group. Secondly, histological analysis of H&E staining showed clearly the boundary between the epidermal layer and the dermal layer, the border of the hair follicle, and the hair follicle cells in the DNCB-DM+T group than in the DNCB-DM group. Third, the gene related to the hair cycle through transcript analysis of total RNA is as follows. In the DNCB-DM group, the mRNA amount of G protein-coupled receptor(Gprc5d), msh homeobox 2(Msx2), patched 2(Ptch2), wingless-type MMTV integration site family(Wnt10b), desmoglein4(Dsg4), keratin associated protein 4-16(Krtap4-16), homeobox C13(Hoxc13), adenomatosis polyposis coli down-regulated 1(Apcdd1) and SRY(sex determining region Y)-box 21(Sox21) were decreased compared with the those of control group. Fourth, the expression of melanin biosynthesis-related genes in the DNCB-DM group was decreased in mRNA amount of tyrosinase (Tyr), oculocutaneous albinism II (Oca2), tyrosinase-related protein 1 (Tyrp1) and dopachrome tautomerase(Dct), in contrast, it was increased in the DNCB-DM+T group than in the control group. Fifth, gene expression profiles of DNCB-DM+T group by analyzing transcripts of isolated total RNA were analyzed and mRNA levels of G protein-coupled receptor(Gprc5d), desmoglein 4(Dsg4), keratin associated protein 4-16(Krtap4-16), patched 2(Ptch2), homeobox C13(Hoxc13), forkhead box Q1(Foxq1), forkhead box N1(Foxn1), and adenomatosis polyposis coli down-regulated1(Apcdd1) were increased compared to the DNCB-DM group. In conclusion, tannin helps to improve symptoms of contact dermatitis, and changes in hair-related gene expression may help to improve the cycle or growth impediment of hair caused by dermatitis.