우엉뿌리와 황칠나무잎 발효액 배합이 C57BL/6 mouse의 모발성장에 미치는 영향

Abstract

ABSTRACT

The Influence in the Mixture of Fermented Liquid in Arctium lappa L. Root and Dendropanax morbifera Lev. Leaf on Hair Growth of C57BL/6 mice Hee-la Ko Advisor : Jung-heon Lee, Ph.D. Department of Beauty and Cosmetic Graduate School of Industry Chosun University

The industrial and cultural development in modern times has many impacts on life, thereby being beneficial and simultaneously causing many diseases and stress in modern people's mind and body. One out of it can be taken hair loss. The hair loss was regarded as a phenomenon of being shown in men whose genetic factor is strong or a phenomenon of appearing due to aging in the past, but is being expanded gradually into diverse forms these days up to women, adolescence, juveniles, and children. As for modern people who pursue young and beautiful life with delaying a change in body and appearance in the more life extension in a human being, the hair loss is positioning as an important element among those things of being desired to be further prevented. An interest in hair loss is now being expanded day by day. A lot of products and therapeutic methods are being developed. But it is the real situation of failing to have a satisfactory result with a treatment outcome. Accordingly, people are growing who aim to keep and prevent scalp health from side effects in the treatments of chemical drugs or physical surgical methods. Thus, this experiment was conducted in order to research into possibility as a material through the fermentation of a natural substance. This experiment was used natural resources available for being approached easily around. First of all, Arctium lappa L. Root is a plant that is used as a food material and is progressed many researches on food. However, a research as a functional material is in the real situation of being insignificant. In terms of a research on a material owing to a reason of safety such as toxicity even if Dendropanax morbifera Lev. Leaf is known to have efficacy as a medicinal plant, this study was carried out in order to be helpful for the development in a hair-loss product through the fermentation of using enzyme with being harmonized the safety in a material, the efficacy in each material, and the nutritive substance according to fermentation. An experimental method divided a mouse into the experimental groups with 3 groups in Arctium lappa L. Root extract group, Dendropanax morbifera Lev. Leaf extract group, and the fermented liquid of Arctium lappa L. Root and Dendropanax morbifera Lev. Leaf, the positive control group with minoxidil 3%, and the control group with a solvent group and a non-treatment group. Hair growth was observed with applying each specimen by 2cc for 5 weeks from Monday to Friday after shaving the dorsal hair. The macroscopic hair growth was observed and shot on the 0th, the 7th, the 14th, the 21st, the 8th, and the 35th day. The inner part of percutaneous was made a macroscopic observation by extracting the dorsal percutaneous after being sacrificed. A structural observation was made with an optical microscope following Hematoxylin & Eosin (H&E), Touidin Blue dye. A antioxidant test and a toxicity experiment in specimen were additionally made. In consequence of observing the mouse's appearance at the macroscopic level in order to figure out hair growth level as the research outcome, the hair growth was observed to be made in minoxidil 3% as the positive control group, and in order of the mixed fermentation between Arctium lappa L. Root and Dendropanax morbifera Lev. Leaf, the fermented Dendropanax morbifera Lev. Leaf, and the fermented Arctium lappa L. Root, which conform to the experimental group. In a medial percutaneous effect, the skin color of the inner part in the back was shown to be darker in order of the group with the mixed fermentation between Arctium lappa L. Root and Dendropanax morbifera Lev. Leaf, the group with the fermented Dendropanax morbifera Lev. Leaf, and the group with the fermented Arctium lappa L. Root, as the experimental group. The tissue that was applied minoxidil 3% as the positive control group for Hematoxylin & Eosin (H&E), and the mixed fermentation liquid between Arctium lappa L. Root and Dendropanax morbifera Lev. Leaf as the experimental group, could be confirmed to be indicated a form of follicle in which number of follicles increases and in which even a form of follicle grows to the surface of skin. The tissue that was applied minoxidil 3% as the positive control group and the mixed fermentation liquid of Arctium lappa L. Root and Dendropanax morbifera Lev. Leaf as the experimental group could be identified to be reduced number of obese cells in the Touidin Blue dyeing. This experiment results in failing to reach a macroscopic hair-growth effect in minoxidil 3%, which corresponds to the positive control group. But the fermented-and-mixed liquid in two natural substances show higher hair growth than each specimen in the hair growth of the mouse. Hence, the fermentation and the mixture in natural objects are judged to give a help to the influence of hair growth. It is considered to be likely a value of application as data to a research of efficacy using the combination of other natural substances or the fermentation using enzyme or in the development of a material in a hair-loss product.