C57BL/6 mice에서 염화아연 및 황산 아연을 이용한 발모 촉진 효과

Abstract

Hair-growth promoting effect of ZnCl₂and ZnSO₄solution in C57BL/6 mice model

Jean-Ok Yu Advisor: Hyun-Jae Shin, Ph.D. Department of Beauty and Cosmetic Graduate School of Industry Technogy Convergence Chosun University

Recently, with the increase of patients with hair loss and the rise of its social cost, various studies have been discussed the method for the treatment. There seems to be some limits in terms of methodology. These methods are apt to use natural extract or to rely on the external factors such as hair transplantation. That's why this study took notes of the effects of zinc as vital nutrition component mineral in the human body and we conducted the experimental study on the method applied to skin to make difference with other methods. These days most studies on the hair loss are made on the basis of natural extracts. However, the results are unsatisfied and the treatments using Minoxidil and Propecia reveal some limitations in that they are effective only when they are maintained continuously. Therefore, despite shortages of the study of hair loss treatment with zinc, it is to be understood as a new attempt. This thesis considers if there are any connection between the zinc known as essential trace elements and the hair growth. Through experiments and studies this paper will determine the potential of the zinc as a therapeutic agent for alopecia. Several studies using zinc about the hair loss are how to take tiny amounts of zinc. That method using zinc for the hair loss treatment is a method of taking a tablet. In this study, by the way, the problem is specific only to the cases of hair loss patients caused by zinc deficiency. This study was carried out to see if sulfate of zinc and zinc chloride have effect in growth of hair in mice and to review the possibility of zinc as hair loss treatment system through that. Therefore, we selected the experimental method of the coating to be applied to the case of hair loss patients with non-zinc deficient. This study is not for the purpose of immediate development of therapeutic zinc, but for searching the power of hair growth and reconsidering the utility value of zinc. The fact that zinc is mainly related to the immune and sex hormone and growth hormone is known through previous research. In particular, it was found that zinc especially inhibits DHT works on the hair follicle, is involved in the activity of the anti-hair loss and hair follicle cells. And it has an effect on hair growth to some extent. Hence, it is considered that supplying zinc through the skin would be effective on hair growth. This experiment was conducted over a total of 6 weeks after epliating the dorsum of the C57BL/6 mouse's hair. it was performed by applying 2 ml to C57BL/6 mouse every day. Each test solution was mixed by 20 ppm ZnSO₄ and ZnCl₂ with purified water. We only used visual observation excluding other observations in the experiment. It was turned out that by visual observation hair growth effects of the mice had been made relatively homogeneous case when hair growth had progressed and since then the hair growth effect had been evaluated as stable, too. Meanwhile an experimental skin tissue was cut in a transverse plane in order to analyze a morphological change in five weeks. As the result, the hair follicles and hair growth condition, hair papilla and hair root well-developed were observed. This result means that the coated zinc on the skin has been absorbed in hair follicles, induces the production of the capillary, and increases in the melanin pigment cells related to enable the hair-regrowth by having influence on growth hormones around the hair follicle. It is believed that people will reconsider our study -the possibility of connection between zinc and for hair loss treatment methods- and especially people would be able to develop creamed or formed hair loss treatment using a small amount of minoxidil zinc solution. In particular, it should be noted the point that it just was the significance of this research and experiment in that it could be applied to the case other than hair loss due to zinc deficiency by choosing directly applying to the skin rather than taking zinc. But this study would be more meaningful with the methods, the regulation of zinc through oral administration or intake of dietary constant, the control of serum zinc concentrations in the body. if the experiment were carried out with a further more in-depth research such as the additional clinical trial, the potential of zinc would be considered as a therapeutic agent for alopecia.