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- Hizikia fusiforme polysaccharides Anti-obesity separation
- Hizikia fusiforme, a brown algae, is a major polysaccharide composed of alginate and fucoidan. In this study, the extraction of the useful substance using the organic solvent is generally avoided and a simple biocompatible / eco-friendly extraction method is devised to obtain an extract containing the water-soluble polymer polysaccharide as the main component from the dried or raw Hizikia fusiforme. In this study, the water soluble polymer polysaccharides were prepared with enzyme digestion and recovered with ethanol precipitation. The changes of reducing sugar content and protein content were observed and the molecular weight distribution was analyzed after enzyme digestion. Molecular weight measurements were performed using HPAEC and HPLC to reveal the constituents of the fraction material having anti-obesity bioactivity. The molecular weight (MW) of the extract was analyzed using a RI (Refractive Index) detector using a GPC column (PSS Suprema 1,000Å). Dextran (MW: 5, 12, 50, 150, 670 kDa) was used as a standard. As a result, polysaccharides composed of fucose, galactose and xylose (Average molecular weight: 18.5 kD) were identified. The IC50 value was determined using an enzyme extract having the best inhibition rate in the anti-obesity activity inhibition experiment. viscozyme, celluclast and alcalse were 14.06, 11.27, and 10.02 mg/ml, respectively, indicating that alcalase is most suitable.
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