6-gingerol이 소포체 스트레스에 의한 인간 전립선암 세포사멸에 미치는 효과

Abstract

Six-gingerol, one of the ginger (Zingiber officinale) compounds, has been shown to cause anti-tumor effects in a variety cancer cells including non-small cell lung cancer cells. Also, it previously reported that 6-gingerol inhibits cell proliferation in human breast cancer cells. Apoptosis is a major mechanism to eliminate cancer cells. The activation of caspase family and endoplasmic reticulum (ER) stress-mediated apoptotic pathway in the key step in apoptosis. The accumulation of unfolded or misfolded protein in ER can induce ER stress. To combat the deleterious effects of ER stress, cells have evolved various protective strategies, activate signal transduction pathways that trigger a complex transcriptional and translational response known as the unfolded protein response (UPR). In this study, cells were treated with the doses of 6-gingerol for a variety time. 6-gingerol induced time -dependent reduced of cell viability in DU145 cells. Expression of cleaved caspase-3, capspase-4, and PARP protein involved in apoptotic cell death were increased in cell treated with 10μM 6-gingerol and shown to the typical apoptotic morphology of fragmentation and condensation after staining with Hoechst 33342. Also, GRP78, p-eIF2α, and CHOP expression were increased in DU145 cells. caspase-3 and NF-κB activity was significantly increased time-dependent in cell treated with 6-gingerol. Apoptosis-related protein, ER stress-related protein, caspase-3, and NF-κB activity were decreased expression in cell treated with PBA. In conclusion, cells have shown that 6-gingerol induces apoptosis through ER stress in DU145 prostate cancer cells.