예쁜꼬마선충(Caenorhabditis elegans)을 이용한 망간항성성이 병원균 감염에 미치는 영향에 대한 연구

Abstract

ABSTRACT

예쁜꼬마선충(Caenohabditis elegans)을 이용한 망간항상성이 병원균 감염에 미치는 영향에 대한 연구

Ju Lee Sun Advisor : Prof. Hoon Jeong Cho Major in Biology Education

Nramp(Natural resistance-associated macrophage protein) is a manganese transporter in macrophage functionally conserved in bacteria and eukaryotes. Manganese(Mn), which is required in small amounts functions as a cofactor for most antioxidants, and also known as a superoxide scavenger. It is also known that mouse defective in Nramp is susceptible to pathogen. Three Nramp homologues, smf-1, smf-2, and smf-3, have so far been identified in the nematode, Caenorhabditis elegans. GFP promoter assay revealed intestinal expressions of smf genes from early embryonic stages and these expressions were maintained through the adult stages. smf deletion mutants have sensitivity to excess manganese. Interestingly, defective smfs cause hypersensitivity to the pathogen, Staphylococcus aureus but not to the non-pathogenic Bacillus subtilis, and their sensitivity were recovered by manganese feeding or knock down of a Mn homeostasis related gene, the Golgi calcium/manganese ATPase, pmr-1, indicating that manganese uptake is essential for the innate immune system. We propose that C. elegans intestinal lumen provide a good and a simple model for macrophage phagosome especially in Mn-mediated innate immune systems.