GC-MS를 이용한 국화과 식물의 휘발성 향기성분 비교

Abstract

Compositae plants are widely distributed over the world, and about 920 genus, 20,000 species are known, and the number of compositae plants native of or naturalized in Korea is about 300. Countries that eat compositae for food are Korea, China and Japan, and they are known to be effective against colds, stomaches, dizziness, high blood pressure and headaches, and anti-bacterial, anti-cancer, and virus suppression. Various studies are being conducted on compositae plants, including research on whitening cosmetics' functionalities using flowers, research on inhibitory effects of cancer cell proliferation, and antioxidant effects of flower extracts. Currently, Aster yomena (kitam). Honda, Aster tataricus Linne Fil, and Chrysanthemum zawadskii Var. latilobum KITAMURA are called by the same name because of their similar appearance and are difficult to distinguish unless they are experts. Therefore, this study was intended to prepare an analytical analysis method to provide basic data onto the volatile aromatic ingredients of Aster yomena (kitam). Honda, Aster tataricus Linne Fil, and Chrysanthemum zawadskii var. latilobum KITAMURA, which are the raw ingredients of similar-looking foods. Among Compositae plants, moxibustion with the same order and an ant with a different order is called ‘dulgukhwa’ because of its similar appearance. As the value of natural and organic foods increases, the demand and production of various food groups increases, and Compositae plants are also in the spotlight in terms of functionality. Therefore, in this study, we plan to conduct research to analyze the volatile aromatic ingredients of Aster yomena, Aster tataricus, and Chrysanthemum zawadskii , which are similar in appearance, and to provide them as data of volatile aromatic ingredients. The volatile aromatic component of Compositae plants was analyzed by GC-MS using SDE and SPME extraction method. Separated volatile aromatic compounds were established and sympathized with the retention index and quantified using n-butylbenzenene added as an internal standard substance. In SDE extraction, a total of 117, 115 and 120 volatile compounds were detected in Aster yomena, Aster tataricus and Chrysanthemum zawadskii, individually, whereas their total amount of volatile compounds were 2,199.49 mg/kg, 1,101.42 mg/kg and 6,682.62 mg/kg, respectively. In SPME extraction, a total of 63, 60 and 83 volatile compounds were detected in Aster yomena, Aster tataricus and Chrysanthemum zawadskii, individually, whereas their total amount of volatile compounds were 713.04 mg/kg, 357.60 mg/kg, 1,101.42 mg/kg and 4,476.31 mg/kg, respectively. As a result, there was not much difference between Aster yomena and Aster tataricus, but Chrysanthemum zawadskii was found to have a difference in content and composition with Campanulales. This study confirmed that the composition and contents of the volatile flavor compounds vary between different extraction method. There were also differences in the patterns of each functional group and terpene type, and it is believed to be available as basic data for volatile aromatic ingredients of Compositae plants.|식물은 부위에 따라 생리활성에 차이가 있으며 국화과 식물의 경우 건강기능성 생리활성에 관한 연구로 꽃에 한정되어왔다. 꽃보다 수확량이 많은 잎을 기능성 소재로 사용할 수 있다면 경제적인 식물소재로 활용할 수 있으며, 재배농가의 소득을 증가시킬 수 있을 것으로 사료된다. 이러한 국화과 식물들 중 외형이 유사한 쑥부쟁이, 개미취 및 구절초의 경우 비슷한 생김새로 인해 일반 소비자들은 외형적으로 구별하는데 어려움이 있다. 따라서 본 연구는 국화과 식물 중 외형이 유사한 쑥부쟁이, 개미취 및 구절초 잎의 휘발성 향기성분을 분석하여 비교하였으며 휘발성 향기성분의 기초자료로 제공하기 위해 연구를 수행하였다.