대장암 생쥐 모델에서 철분이 광역학 치료에 미치는 효과
- Author(s)
- 박성남
- Issued Date
- 2020
- Keyword
- Photodynamic therapy, Iron, photolon, CT26 cells
- Abstract
- Photodynamic therapy (PDT) is a noninvasive cancer treatment method that treats cancer using photosensitizing agents and light. However, PDT needs to be developed to increase the efficiency of treatment due to the limitation of light transmission and the limited effect of photosensitizers. This study was conducted to observe the effect of iron on the effects of PDT on cancer treatment. The experimental group was divided into four groups: control group, iron group, PDT group, and Iron+PDT group. The CT26 tumor bearing mouse model was used to induce carcinoma by injecting colon cancer cells (CT26 cells) into Balb/c mice. Iron (10 mg/kg BW) was injected intraperitoneally 2 hours prior to photon injection. Photolon (2.5 mg/kg BW) was intraperitoneally injected 2 hours prior to laser irradiation. The laser was irradiated to the tumor site with 80J dose using a semiconductor laser.
The tumor volume was measured every three days after laser irradiation using digital caliper. After three hours of laser irradiation, the gene expression was measured by the mRNA sequencing method and the cancer tissue lipid peroxid(TBARS) content was measured by using OxiSelect™
TBARS Assay Kit. The volume of tumors was increased by 421% on the 14th day compared to the 0 day, the control group was increased by 418%, the PDT group was increased by 114%, and the Iron+PDT group was reduced by 53%. After 3 hours of PDT, the TBARS content of tumor tissue was not different between control and iron group. Tumor tissue TBARS content increased 178% over control group, while Iron+PDT group increased 67% over PDT group.
Three hours after PDT, gene expression in tumor tissue was measured and 12 chemotaxis-related genes in the Iron+PDT group were increased by 17.0 times, 13 genes related to cytokin-cytokine receptor interaction increased by 7.3 times, and the corresponding gene response was increased by 10 times, respectively.
In these experiments, when photolon-PDT was performed using CT26 tumor bearing mouse, iron administration significantly reduced the volume of tumors, increased the TBARS volume in cancer tissues, and increased the expression of genes related to inflammatory reactions.
The anticancer effect increased by iron in Photolon-PDT is considered to be able to use iron very effectively in the treatment of cancer by PDT.
- Alternative Title
- Effects of Iron on Photodynamic Therapy of Cancer in Colon Cancer Bearing Mouse Model
- Alternative Author(s)
- Park Seong-Nam
- Affiliation
- Department of Medicine, Graduate School of Chosun University
- Department
- 일반대학원 의학과
- Advisor
- 안태규
- Awarded Date
- 2020-02
- Table Of Contents
- 1. 서론 .....................................1
A. 대장암 .......................................1
B. 광역동 치료 ................................1
C. 광민감제 ....................................3
D. 빛(광원) ....................................4
E. 반응성 산소 ................................5
F. 금속이온 ....................................5
G. 실험목적 ...................................6
2. 재료 및 방법 .............................8
A. 실험재료 .....................................8
B. 실험동물 .....................................8
C. 세포배양 .....................................8
D. Colon cancer bearing mouse model 확립 ........9
E. 광역동치료(PDT) ..............................9
F. 종양조직 TBARS 측정 .........................10
G. 종양 조직 RNA-sequencing 및 분석 ............10
H. 통계처리 ....................................11
3. 결과 ....................................12
A. CT26 cancer bearing mice의 종양에 PDT 시행 후 종양 용적의 변화 .........................12
B. CT26 cancer bearing mice의 종양에 PDT 시행 후 종양조직 TBARS 량의 변화 .................12
C. PDT 군과 Iron+PDT 군의 암조직 유전자 세트 (Gene set) 발현 변화 ........................13
D. PDT군과 Iron+PDT군의 암조직 화학주성 (chemotaxis)관련 유전자 발현 변화 ...........13
E. PDT군과 Iron+PDT군의 암조직 사이토카인과 사이토카인수용체 상호반응(cytokine-cytokine receptor interaction)관련 유전자 발현 변화.............................................14
F. PDT군과 Iron+PDT군의 암조직 사이토카인 활성 (cytokine activity)관련 유전자 발현 변화.............................................14
G. PDT군과 Iron+PDT군의 암조직 염증반응 (inflammatory responsec)관련 유전자 발현 변화.........................................15
4. 고찰 ....................................16
5. 요약 ....................................22
6. 참고문헌 ..............................24
- Degree
- Doctor
- Publisher
- 조선대학교 대학원
- Citation
- 박성남. (2020). 대장암 생쥐 모델에서 철분이 광역학 치료에 미치는 효과.
- Type
- Dissertation
- URI
- https://oak.chosun.ac.kr/handle/2020.oak/14112
http://chosun.dcollection.net/common/orgView/200000284886
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Appears in Collections:
- General Graduate School > 4. Theses(Ph.D)
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