대장암 생쥐 모델에서 철분이 광역학 치료에 미치는 효과

Abstract

Photodynamic therapy (PDT) is a noninvasive cancer treatment method that treats cancer using photosensitizing agents and light. However, PDT needs to be developed to increase the efficiency of treatment due to the limitation of light transmission and the limited effect of photosensitizers. This study was conducted to observe the effect of iron on the effects of PDT on cancer treatment. The experimental group was divided into four groups: control group, iron group, PDT group, and Iron+PDT group. The CT26 tumor bearing mouse model was used to induce carcinoma by injecting colon cancer cells (CT26 cells) into Balb/c mice. Iron (10 mg/kg BW) was injected intraperitoneally 2 hours prior to photon injection. Photolon (2.5 mg/kg BW) was intraperitoneally injected 2 hours prior to laser irradiation. The laser was irradiated to the tumor site with 80J dose using a semiconductor laser. The tumor volume was measured every three days after laser irradiation using digital caliper. After three hours of laser irradiation, the gene expression was measured by the mRNA sequencing method and the cancer tissue lipid peroxid(TBARS) content was measured by using OxiSelect™

TBARS Assay Kit. The volume of tumors was increased by 421% on the 14th day compared to the 0 day, the control group was increased by 418%, the PDT group was increased by 114%, and the Iron+PDT group was reduced by 53%. After 3 hours of PDT, the TBARS content of tumor tissue was not different between control and iron group. Tumor tissue TBARS content increased 178% over control group, while Iron+PDT group increased 67% over PDT group. Three hours after PDT, gene expression in tumor tissue was measured and 12 chemotaxis-related genes in the Iron+PDT group were increased by 17.0 times, 13 genes related to cytokin-cytokine receptor interaction increased by 7.3 times, and the corresponding gene response was increased by 10 times, respectively. In these experiments, when photolon-PDT was performed using CT26 tumor bearing mouse, iron administration significantly reduced the volume of tumors, increased the TBARS volume in cancer tissues, and increased the expression of genes related to inflammatory reactions. The anticancer effect increased by iron in Photolon-PDT is considered to be able to use iron very effectively in the treatment of cancer by PDT.