ETV2에 의한 혈관평활근세포 이동 기전 규명

Abstract

The ETS (E-twenty six) family of transcription factors consist of 12 subfamilies. Among the ETS factors, ETV2 is transiently expressed and known to play a key role in angiogenesis during embryonic development. However, it is not expressed in the adult. Recently, ETV2 has been found that damaged tissues of the adult are expressed for improved vascular regeneration and remodeling. In this study, the role of ETV2 was confirmed in the process of migration and proliferation vascular smooth muscle cells (VSMCs), the cause of arteriosclerosis and restenosis. First, ETV2 adenoviral vectors were constructed, VSMCs in ETV2 overexpression and inhibitor were identified, MMP9 upregulation by ETV2 overexpression and MMP9 downregulation by silencing were confirmed. In addition, ETV2 overexpression shown the phenotype change in VSMCs, as the expression level of contractile maker α-SMA, Myocd were decreased, while the expression level of synthesis maker Connexin43 was increased, the confirming the effect of phenotype control of VSMCs by ETV2 regulation. Furthermore, the expression of ETV2 was confirmed in rat model with balloon injury and VSMCs specificity. In conclusion, the present result demonstrated that ETV2 activated MMP9 induce cell migration in VSMCs. Furthermore, Effect of ETV2 overexpression has necessary to study in detail the effects on VSMCs.