효소 고정화 기법을 이용한 환경호르몬 Bisphenol A (BPA) 검출 바이오센서 개발

Abstract

Bisphenol A (BPA) was used in raw materials to make polycarbonate or epoxy resins and used extensively in everyday life. The 300 tons of BPA was produced and consumed per year. Early studies have shown that BPA is an endocrine system disruptor that the same action and estrogen from the core of the body. Endocrine system disruptors act as the endocrine hormone and give the confusion in the endocrine system. BPA is an endocrine disruptor that acts like an estrogen in woman's body and also have a detrimental effect on child behavior and learning. BPA is a substance that causes interference even at low concentrations. However, BPA in the plastics, canned, and receipts was used in a variety of locations and is easily exposed to daily life. Since BPA is absorbed even by contact with the skin, it needs to be deal with very carefully. In this study developed a BPA detection system using biosensor immobilized with laccase. The detection was realized by immobilizing laccase on the working electrodes which are Multi-walled Carbon Nanotubes (MWCNTs) and Polyaniline (PANI). When laccase was immobilized on the surface of working electrode, the sensing activity showed differences in accordance with the immobilizing method. After immobilizing the enzyme on the electrode, cyclic voltammetry was measured with the changes of BPA concentrations. The electrode potential was driven in the cathodic direction from 2 to -2 V at scan rate of 300 mV/s. In order to increase the sensitivity of the biosensor, experiments were carried out with the addition of dyes which function as electron transfer mediator at the stage of immobilization. Toluidine blue, Bromothymol blue and Reactive red 120 were used as the intermediate dyes. The linear range of biosensor was from 1 ppb to 10 ppm and the detection limit was as low as 0.01 ppb. Amperometry was measured to observe the change in current value due to BPA concentration change.