타크로리무스로 유발된 급성 신독성 흰쥐에서 녹차추출물과 제미글립틴의 신보호 효과

Abstract

Background : It has been reported that the proteinuria is an early predictive marker in detection of tacrolimus (TAC) nephrotoxicity. The aim of this study was to investigate the renoprotective effects of green tea extract (GTE) and dipeptidyl peptidase IV (DPP IV) inhibitor gemigliptin on TAC-induced acute nephrotoxicity in mice.

Methods : The mice (n=30) were divided into 5groups (n=5/group); control group were intraperitoneally (IP) injected 0.9% saline, TAC group were IP injected TAC2 mg/kg, DPP IV inhibitor group were given inadditiongemigliptin20 mg/kg (G20) by oral gavage. TAC-GTE group were given TAC by IP injection and GTE 100 mg/kg by subcutaneous injection. TAC-GTE-G20 group were given with same dosages.

Results : The 24 hours urine protein amounts were significantly increased in TAC group compared to control group and significantly decreased in TAC-GTE-G20group compared to TAC group. The nitric oxide (NO) production by TAC was significantly suppressed by EGCG and gemigliptin in management. Renal tissue malondialdehyde level was significantly increased in TAC group compared to control group and significantly decreased in TAC-EGCG-G20 group compared than that of TAC group. The renal function and antioxidant enzyme activities were significantly suppressed in TAC group compared with control group and restored in EGCG and gemigliptin treatment group.

Conclusion : GTE and gemigliptin treatment has beneficial antiproteinuric and renoprotective effects on TAC-induced acute renal injury in mice.