사람 하인두 편평암세포 FaDu에서 caspase 의존적 세포사멸을 통한 Anthricin의 항암활성

Abstract

Anthricin(Deoxypodophyllotoxin), a naturally occurring flavolignan, has well known anti-cancer effect in several cancer cells such as prostate cancer cell, cervical carcinoma cell and pancreatic cell. However, in oral cancer, the anti-cancer effect of Anthricin is currently indeterminate. Here, we examined the anti-cancer effect and mechanism of action of Anthricin in human FaDu hypopharyngeal squamous carcinoma cells. Our data showed that Anthricin inhibits cell viability in a dose- and time-dependent manner and MTT assay and Live & Dead assay by IC50 value of 50nM. Inaddition, Anthricin treated FaDu cells markedly induced apoptosis by DAPI stain and FACS. Furthermore, Anthricin activates anti-apoptotic factor such as caspase-3, -9 andpoly(ADP-ribose) polymerase(PARP), suggesting that caspase-mediated pathways are involved in Anthricin-induced apoptosis. Anthricin treatment accumulated pro-apoptotic factor, Bax, followed by inhibition of cell growth. Taken together, these results indicated that Anthricn-induced cell death of human FaDu hypopharyngeal squamous carcinoma cells was mediated by mitochondrial-dependent apoptotic pathway. In summary, our findings provide a framework for further exploration on Anthricin as an novel chemotherapeutic drugs for human oral cancer cells.