Tryptic soy-serum-bacitracin-vancomycin 배지에서 배양된 Aggregatibacter actinomycetemcomitans 이외의 세균 동정
- Author(s)
- 조어진
- Issued Date
- 2016
- Abstract
- he aim of this study was to identify the non-Aggregatibacter actinomycetemcomitans bacteria grown on the tryptic soy-serum-bacitracin-vancomycin (TSBV) medium, an A. actinomycetemcomitans selective medium. A total of 82 unidentified bacterial isolates from the oral cavities of a Korean population were kindly provide by the Korean Collection for Oral Microbiology. All the clinical isolates were grown on TSBV medium and bacterial DNA purified from each isolate was subjected to PCR with universal primers specific for bacterial 16S rRNA genes (16S rDNAs) sequence. The each bacterial 16S rDNA was amplified by PCR and the nucleotide sequences of it was determined by the dideoxynucleotide chain termination method. They were identified by 16S rDNA sequence comparison method at the specie-level. In this study, we used 98.7% 16S rDNA gene sequence similarity as the threshold for differentiating two species. The data showed that Neisseria spp. (42 strains). Fusobacterium spp. (10 strains), Capnocytophaga spp. (8 strains), Propionibacterium acnes (5 strains), Aggregatibacter aprophilus (4 strains), Campylobacter spp. (5 strains), Veillonella dispar (3 strains), Streptococcus sp. (1 strain), Haemophilus parainfluenzae (1 strain), Leptotrichia wadei (1 strain), Morococcus sp./Neisseria sp. (1 strain), and Staphylococcus sp. (1 strain) were identified. These results could be used to develope a new A. actinomycetemcomitans-selective medium which is more effective than the TSBV medium in future studies.
- Alternative Title
- Identification of non-Aggregatibacter actinomycetemcomitans bacteria grown on the tryptic soy-serum-bacitracin-vancomycin medium
- Alternative Author(s)
- Jo, Eo-Jin
- Affiliation
- 조선대학교 치과대학원
- Department
- 일반대학원 치의학과
- Advisor
- 국중기
- Awarded Date
- 2017-02
- Table Of Contents
- ABSTRACT x
I. 서 론 1
II. 연구재료 및 방법 4
1. 세균 및 세균배양 4
2. 16S rDNA 클로닝 4
3. 16S rDNA 핵산염기서열 결정 및 상동성 검색 5
4. 계통발생 분석 5
5. 신종가능성 균주들의 최적성장조건 결정 6
Ⅲ. 연구 결과 9
1. 구강 병소에서 분리된 세균 동정 9
2. 동정된 세균들의 계통발생 분석결과 9
3. 신종 가능성 균주들의 최적성장조건 결정 10
Ⅳ. 총괄 및 고안 23
Ⅴ. 결 론 27
Ⅵ. 참 고 문 헌 29
- Degree
- Doctor
- Publisher
- 조선대학교 치과대학원
- Citation
- 조어진. (2016). Tryptic soy-serum-bacitracin-vancomycin 배지에서 배양된 Aggregatibacter actinomycetemcomitans 이외의 세균 동정.
- Type
- Dissertation
- URI
- https://oak.chosun.ac.kr/handle/2020.oak/13042
http://chosun.dcollection.net/common/orgView/200000265923
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Appears in Collections:
- General Graduate School > 4. Theses(Ph.D)
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- Embargo2017-02-21
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