와송(Orostachys japonicus A.)의 기내배양과 유용물질의 생리활성에 관한 연구

Abstract

In the tissue culture using leaf tissue of Orostachys japonicus, callus were induced most efficiently in 5-week-after culture in the MS medium supplemented with 10 μM BAP and 4 μM 2,4-D at the growth condition of 24℃ 6500Lux 16 hr-day-length. Adventitious roots developed in all the medium supplemented with 0, 0.1, 1 μM 2,4-D whereas roots formed only in the uppersides of the leaflets not in contact with MS medium containing 0.5 μM BAP and 100 μM 2,4-D. Shoot regeneration was observed when callus induced in the MS medium containing 10 μM BAP and 4 μM 2,4-D was transplanted in to the MS medium without any plant growth regulators. Massive proliferation methods given by these tissue culture methods for O. japonicus might provide efficient ways to its production. The ethylacetate fraction extract for O. japonicus contained 634.48 μg/㎖ polyphenol and 205.20 μg/ml flavonoid. The polyphenol and flavonid contents of O. japonicus were 0.16x and 3.3x higher than those of Aronia a source of well-known health supplement food, respectively. The ABTS radical scavenging ability of ethylacetate fraction extract at 1 mg/ml was higher than 95% which is comparable to ascorbic acid of 97%. The APX enzymatic activity and catalase activity were 1125.89 μmol ascorbate oxidized/min/mg protein and 119.87 H2O2 decomposed/ min/mg protein, respectively. In disc agar plate diffusion assay, the extract gave rise to a larger inhibition circle with Lissteria monocytogenes, Staphylococcus epidermidis, Staphylococcus aureus and Malassezia furfur strains compared with antibiotics kanamycin suggestive of high antibiotic activity. These results suggest that fraction extract of O. japonicus might be very effective and economical in developing natural antioxidant and antibiotic.