세발나물 분말이 지방세포와 고지방식이 유도 비만 흰쥐에 미치는 영향

Abstract

ABSTRACT

Effects of Spergularia marina Griseb Powder on Adipocytes and High-Fat Diet-Induced Obese Rats

by. Park Yong Hyun Advisor : Prof. Lee, Jae Joon, Ph. D. Department of Food and Drug, Graduate School of Chosun University

In this study, it was carried out to investigate the physiological activities, lipid metabolism and anti-obesity effects of Spergularia marina Griseb, a kind of the halophytes. In vitro, the anti-obesity effects of Spergularia marina Griseb ethanol extracts were examined by inhibitory action on proliferation and differentiation of 3T3-L1 preadipocytes and pig preadipocytes. In further, the effects of Spergularia marina Griseb powder, on lipid metabolism and anti-obesity in rats fed a high fat diet, were evaluated, in vivo.

Firstly, this research was to analyze proximate compositions and dietary fiber of the Spergularia marina Griseb. The proximate compositions of Spergularia marina Griseb, as a dry matter basis, were 4.10% of moisture, 32.47% of crude protein, 4.53% of crude fat, 20.68% of crude ash and 38.22% of carbohydrate. Total polyphenol content of Spergularia marina Griseb ethanol extract were found 68.04 mg/g in 1,000 ppm. Total flavonoid content of Spergularia marina Griseb ethanol extract were found 28.60 mg/g in 1,000 ppm.

Secondly, the study was carrid out to determine the effects of the Spergularia marina Griseb on proliferation and differentiation of 3T3-L1 preadipocytes and pig preadipocytes. To measure the cell proliferation and differentiation, cells were treated with 50, 100 and 200 μg/mL ethanol extracts of Spergularia marina Griseb, for two days, and dimethyl sulfoxide(DMSO) was used as the control group. Fifty and 100 μg/mL of the Spergularia marina Griseb ethanol extracts were not significantly different compared to the control group, but 200 μg/mL of the Spergularia marina Griseb ethanol extract significantly decreased the proliferation of 3T3-L1 preadipocytes compared to the control. Spergularia marina Griseb ethanol extracts proved the inhibitory effect on differentiation of 3T3-L1 adipocytes in a dose-dependent manner. One hundred, 200 μg/mL of the Spergularia marina Griseb ethanol extract significantly decreased the triglyceride accumulation of 3T3-L1 adipocytes compared to the control. Two hundred μg/mL of the Spergularia marina Griseb ethanol extracts significantly decreased the proliferation of pig preadipocytes compared to the control. Spergularia marina Griseb ethanol extracts proved the inhibitory effect on differentiation of pig adipocytes in a dose-dependent manner. One hundred, 200 μg/mL of the Spergularia marina Griseb ethanol extract significantly decreased the triglyceride accumulation of pig adipocytes compared to the control group. These actions indicate that the ethanol extracts of Spergularia marina Griseb may have potential to reduce the proliferation, differentiation and fat accumulation of 3T3-L1 preadipocytes and pig preadipocytes.

Thirdly, the effects of Spergularia marina Griseb powder on lipid metabolism and anti-obesity in rats fed a high fat diet were as follows; male Sprague-Dawley rats were assigned to 4 groups : normal diet group(N), high fat diet group(HF), high fat diet with 3% Spergularia marina Griseb(HF-SL), high fat diet with 5% Spergularia marina Griseb(HF-SH). The body weight gain and FER were increased due to a high fat diet, but that were decreased in the HF-SL group compared with the HF group. There was no significant differences in the weights of liver by Spergularia marina Griseb powder administration. Epididymal adipose tissues of HF-SL and HF-SH groups were significantly more decreased than in the HF group. Weights of mesenteric, retroperitoneal, perinenal and total adipose tissues were decreased by the Spergularia marina Griseb powder fed groups, especially HF-SH group was almost decreased to the N group level. Serum ALT and AST activities were signicantly decreased in the Spergularia marina Griseb fed groups compared with the HF group, but serum ALP and LDH activities were no differences with HF group. Level of serum HDL-cholesterol decreased in the HF group, but this level increased significantly in the Spergularia marina Griseb powder fed groups. On the contrary, levels of serum triglyceride, total cholesterol, LDL-cholesterol, as well as atherogenic index and cardiac risk factor were considerably reduced in the Spergularia marina Griseb powder fed groups compared with the HF group. Levels of triglyceride in liver and mesenteric adipose tissue significantly lowered in Spergularia marina Griseb. administered groups than in the HF group. Levels of cholesterol in epididymal adipose tissue and mesenteric adipose tissue significantly lowered in Spergularia marina Griseb administered groups than in the HF group. High fat diet administration were induced the marked accumulation of large lipid droplets and resulted in typical histopathology, but Spergularia marina Griseb administered groups attenuated lipid droplets accumulation in hepatocytes. Fat cell size of epididymal adipose tissue was lower in Spergularia marina Griseb administered groups compared with the HF group. The results suggest that Spergularia marina Griseb powder could make better lipid metabolism of serum, liver and adipose tissue and may decrease lipid storage.