두충나무 수액 및 진액의 항세균 및 항산화 활성 연구

Abstract

Background & purpose

Eucommia ulmoides is a Traditional Chinese Medicine that has been used for longevity, being strengthen the muscles and joints. While traditional application of Eucommia was more focused with its effects on muscles and bones, recent scientific evidence supports the immune, anti-cancer, and anti-hypertensive properties of Eucommia barks and leaves. In practice, it has been shown that various compounds identified from Eucommia such as flavonoids, lignans, and iridoids, exhibit the above medical properties. Furthermore, a new component called “Eucommicin A” was also shown to suppress cancer stem cells.

So far numerous studies have been conducted on the protective effects on hypertension, obesity, osteoporosis, safety assistants of corticosteroids of Eucommia bark and leaves. It was very effective in animal, but did not in humans, except for the blood pressure reduction. In this paper, we changed the experimental method and chose its sap and its liquamen that were not previously studied to further examine the health benefits of Eucommia.

Methods After peeling its bark, we collected the 150 ml of sap, centrif㎍ed it, extracted with Ethyl Acetate, and then again concentrated by vacuum concentrator. The liquamen were obtained by the following method. Without adding any water, to the tree trunk Eucommia 10 ㎏ by heating at a temperature of 105℃ for 24 hours, we got the 2.5ℓ and then in the same as above. If the gutta percha of its bark were to protect the plants from insects and fungus, there is more expectation in the inner trunk, its saps. We measured the following from the collected sap and the liquamen, 1) the DPPH radical scavenging ability and 2) reducing power 3)total phenol content, 4) the cytotoxicity and the nitric oxide production, 5) the antibacterial activity thro㎍h the antimicrobial spectrum, and 6) the component analysis. Result Each result is as follows. 1. As to the radical scavenging ability, in the high concentration (0.05 ~ 1 ㎎/㎖) showed the effect of 30~80% in comparison to the ascorbic Acid. But it was nearly the same as those in the low concentration cases. It is similar to the radical scavenging ability of α-tocopherol. 2. The high concentration of the sap showed 50% performance as to the reducing power in comparison with the ascorbic Acid. It was nearly the same in the low concentration. However, very excellent reducing power was being shown in the case of the liquamen. It is nearly similar the level like the ascorbic Acid in the high concentration(0.5 ㎎/㎖ or more), but in the low concentration less than 0.1, the reducing power was shown more than two times. 3. The total phenol content was not so higher than the gallic Acid, which is the control group. The total phenol content in the liquamen showed a little bit more because of heating for a long time. However, it is regarded that compound having the phenol. 4. As to the cytotoxicity test, the survival rate in the sap was high concentration. However, the cell which becomes extinct was abundant in the liquamen of the high concentration(0.05 ㎎/㎖ or more). It means that the side effect will be able to follow in case of taking this in the high-capacity. 5. As the antibacterial spectrum analysis, They are no reaction in the Gram- negative bacteria – Alcaligenes faecalis ATCC 1004, Salmonella typhimurium KCTC 1925, Escherichia coli KCTC 1923, ESBL P3, ESBL U4, Pseudomonas aer㎍inosa KCTC 1637. But the inhibition zone of the liquamen are wider than the sap in the Gram-positive bacteria – KCTC 1928, MRSA 4-5, MRSA 693E, the VRSA 6. The ingredient of the sap is similar to the vegetable Sterol line. β-sitosterol and Stigmasterol are mainly contained and there seems to be some influence on the decrease of serum cholesterol and anti-cancer, antibiotic, and AD. The ingredient of the sap is similar to the vegetable Sterol line. Conclusion When comparing the sap and the liquamen, it came out of the reducing power and antibacterial activity against super bacteria. But the cytotoxin problem remained, the supplementary research should be guaranteed afterward. The sap and the liquamen were not used in Chinese medicine so far at all, but this study confirms what the sap and the liquamen extract shows the effect like the above. It was clarified they have the effects as much as the bark and leaves thro㎍h this experiment, and it must be regulated a reckless abuse of the liquamen.