상백피의 식물화학적 성분과 항염증 활성 연구

Abstract

The root bark of Morus alba L., called "Sang-Baek-Pi" in Korea, has been used in traditional medicines for the treatment of lung-heat, cough, asthma, hematemesis, dropsy, and hypertension. Previous phytochemical investigations resulted in the isolation of polyphenolic constituents including prenylated flavonoids, benzofurans and Diels-Alder type adducts with various biological activity such as cytotoxicity, antioxidant, cancer cell invasion and migration, and hepatoprotection. In addition, the prenylated flavonoids, main constituents of this plant, have also been shown to exhibit various anti-inflammatory activities. However, the pharmacological evaluation on the minor constituents of the root of M. alba has not been performed in detail, because of its difficulty for the isolation and the presence of trace amount. In the present study, by means of repeated column chromatography using silica gel, Sephadex LH-20, and LiChroprep RP-18, twelve prenylated flavonoids, along with four benzofuran compounds and one triterpenoid were isolated. The structures of the known compounds were identified as kuwanon G (1), kuwanon E (2), oxyresveratrol (3), moracin M (4), mulberrofuran G (5), moracin R (6), maracin O (7), noratocarpanone (8), kuwanon T (9), morusin (10), mulberofuran A (11), mulberofuran B (12), α-amyrin acetate(13), sanggenon A (14), sanggenon M (15), sanggenon F (16), and sanggenol A (17), by comparing their spectroscopic data with those reported in the literature. Among these isolates, the inhibitory activity of eleven compounds against IL-6 production in TNF-α stimulated MG-63 cells was examined. Most of the isolated compounds showed potent inhibitory activity against IL-6 production in TNF-α stimulated MG-63 cells. This thesis reports the isolation and structural characterization of these compounds and their inhibitory activities against IL-6 production.