달맞이꽃종자유와 달맞이꽃종자유-로즈마리혼합오일이 아토피피부염 유발 동물모델에 미치는 효과

Abstract

ABSTRACT

Effect of Evening Primrose Oil Mixed Oil of Evening Primrose-Rosemary on Atopic Dermatitis-Induced Animal Model

Kim, Seon Ju Advisor : Prof. Lee, Mi Ja Department of Complementary and Alternative Medicine, Graduate School of Chosun University

Recently more studies have been focused on atopic dermatitis (AD) due to its rapid increase every year in all generations, especially children. Nevertheless, not a lot known about its mechanism and effective therapeutic agents. It is suggested that aromatherapy using herb oil is very effective in for the treatment of atopic dermatitis. But there are only few experimental reports about the actual effects of or either single compound herb oil. Some studies demonstrated that evening primrose oil, rosemary oil or brending of the two could serve as a putative agent in AD treatment for its antioxidative effect. This study aimed to evaluate the effects of evening primrose oil and the mixed oil of evening primrose-rosemary on AD-induced NC/Nga mice. Experiments were performed to examine not only the changes in their body and organ weights but also sensory assessment and antioxidative effects such as electron donating activity (EDA) and lipid peroxidation activity (LPA) of the compound oil. It also examined the changes in epidermal thickness, IgE level, and the number of mast cells from the tissues of AD-induced NC/Nga mice. As for the result of four different groups were made for this study: a control group, AD-induced group, evening primrose oil-treated group, and evening primrose-rosemary mixed oil-treated group. There were no significant differences in the weights of body and organs among the control group, AD-induced group and oil-treated groups. Oil-treated groups showed significantly sensory assessment and antioxidative effects such as EDA and LPA compared with AD-induced group. As for the changes of epidermal thickness using quantitative assay and light microscopy, both the evening primrose oil-treated group and mixed oil-treated groups showed significantly decrease compared with AD-induced group. The decrease in thickness was especially prominent in the mixed oil-treated group. While both single and compound oil-treated groups showed a significantly decreased IgE level in serum than AD-induced group, and especially mixed oil-treated group. In the degranulation of mast cells by quantitative assay and light microscopy, evening primrose oil- and mixed oil-treated groups showed significant decreased changes of degranulation compared with AD-induced group, and especially mixed oil-treated group. This study is believed to contribute as the utilization value in the aromatherapy field of alternative medicine in AD treatment.

Key Words: Evening primrose oil, Mixed oil of evening primrose-rosemary, Atopic dermatitis, Antioxidative effect, Aromatherapy