갈조류 미역 배우체 세대의 동결보존에 관한 연구

Abstract

The marine brown alga, Undaria pinnatifida (Harvey) Suringar, is edible and an important commercial seaweed in Asian countries. In accordance with global warming and climate changes, the development of methods for genetic preservation and the new cultivar is important. Cryopreservation is an important technology that allows the viable storage of cells of tissues over long periods of time at extremely low temperatures (-196℃) for their use as biological and genetic resources. Successful cryopreservation is often judged by the survival of algal cells and their re-growth as viable cultures or complete plant regeneration. Previous studies in cryopreservation of U. pinnatifida have been focused mainly on damages induced by the freeze-thaw regime than recovery rates of post-cryopreservation. In this study, we evaluated the effects of variable Light-Emitting Diode (LED) sources on the viability of the gametophyte of U. pinnatifida after cryopreservation. Gametophyte of U. pinnatifida was cultured at 20℃ under blue LED (460 nm), red LED (660 nm), green LED (530 nm), and white LED (mixed wavelength) lamps with light intensity of 20, 40, 60 μmol photons·m-2·s-1. After post-cryopreservation, the cell viability of gametophyte was examined every two days during 3 weeks. Cell viability was increased to 95.42 ± 0.9% under blue LED light with light intensity of 40 μmol photons·m-2·s-1. We also estimated pigment contents with in the gametophyte of U. pinnatifida depending on different light quality during 3 weeks. In my result, the pigment contents within gametophyte under blue light were much higher than under other lights. The present result showed that the optimized recovery light is blue (460 nm) with 40 μmol photons·m-2·s-1 after the freeze-thaw.