Tyrosol이 KB 사람 구강암세포의 성장억제에 미치는 영향

Abstract

Tyrosol, a phenylethanoid and a derivative of phenethyl alcohol, possess various biological activities in common such as anti-oxidation and cardioprotective activity. The principal source of tyrosol in the human diet is olive oil. However, the anti-cancer activity of tyrosol has not yet been well defined. In this study, therefore, the cytotoxic activity of tyrosol and the mechanism of cell death exhibited by tyrosol were examined in KB human oral cancer cells.

The cytotoxic effect of tyrosol on cell growth in KB cells was examined by inhibition of cell growth (MTT assay). In addition, the cell death mechanism by tyrosol was examined using DAPI staining, DNA fragmentation analysis and immunoblotting.

1. Treatment of tyrosol in KB cells induced the inhibition of cell growth in a concentration- and a time-dependent manner as determined by MTT assay. 2. Treatment of tyrosol induced the nuclear condensation in KB cells. 3. Treatment of tyrosol induced the DNA fragmentation in KB cells. 4. The proteolytic processing of caspase-3, -7, -8 and -9 was increased by tyrosol treatment in KB cells. 5. The proteolytic processing of PARP was increased by tyrosol treatment in KB cells.

These results suggest that tyrosol can induce the suppression of cell growth and cell apoptosis in KB human oral cancer cells, and therefore, that it may have potential properties for anti-cancer drug discovery.