Dendropanax morbifera Lev. 메탄올 추출물에 의한 사람 구강암 세포 KB의 apoptosis 유도

Abstract

ABSTRACT

Induction of Apoptosis by Dendropanax morbifera Lev. Methanol Extract in KB Human Oral Cancer Cells

Cho, Seon-Ho Advisor: Prof. Kim, Do Kyung, Ph.D. Department of Biodental Engineering, Graduate School of Chosun University

Dendropanax morbifera is evergreen broad-leaved arboreous tree belongs to the araliaceae. The country of origin is mainly the South Korea. The scientific name is Dendropanax morbifera that component is based compound polyacetylenes. Recently, many researchs have been conducted about anti-cancer effects using Dendropanax morbifera in liver cancer, lung cancer, cervical cancer, and so on. However, the effects of Dendropanax morbifera on oral cancer is not clearly established. Therefore, the main goal of this study was to investigate the effect of Dendropanax morbifera extract on cell growth and apoptosis induction in oral cancer cells.

To determine the effects of Dendropanax morbifera on cell growth and apoptosis induction in oral cancer cells, it was examined by inhibition of cell growth (MTT assay), nuclear staining with DAPI, DNA fragmentation analysis determination of caspase activation and immunoblotting in KB human oral cancer cells.

The results were as follows. 1.Dendropanax morbifera methanol(DMME) extract induced the inhibition of cell growth depended on the Dendropanax morbifera extract treatment time and concentration in KB cells. 2.DMME observed the changing nuclear size and nuclear condensation in KB cells. 3. DMME induced the DNA fragmentation in KB cells. 4.DMME promoted proteolytic cleavages of procaspase-3, -7, -8 and -9 with the increases in the amount of cleaved caspase-3, -7，-8 and -9. 5. Cleaved PARP was increased by DMME in KB cells.

These results suggest that Dendropanax morbifera can induce the suppression of cell growth through the cell apoptosis pathways in KB human oral cancer cells, and that it many have potential properties for anti-cancer drug discovery.

KEY WORDS:Dendropanax morbifera, Anti-cancer therapy, Apoptosis, Cell death, KB human oral cancer cells.