구강 및 의치 세정제 조성을 위한 황칠나무 추출물의 항균, 항산화 효과 및 세포 독성에 관한 연구

Abstract

Purpose : The purpose of this study as a preceding research is to assess whether Dendropanax morbifera(D. morbifera) can be used for the development of natural mouthwash and denture cleaner, and to analyze the antimicrobial, antioxidant activity and cytotoxicity of D. morbifera extract. Methods : The extract was obtained from branches of D. morbifera. The solvent fractions were acquired by fractionating D. morbifera extract using hexane, ethyl acetate, and butanol solvent. The composites for mouthwash and denture cleaner were produced by adding D. morbifera extract into saline or chlorohexidine solvent, and 4 types of composites were made. Among the analysis methods for antimicrobial proliferation-inhibiting activity, paper disc test was used to evaluate the antimicrobial and antifungal activity of D. morbifera extract, solvent fractions, and composites for mouthwash and denture cleanser against S. mutans and C. albicans. The analysis of antioxidant activity was carried out through DPPH radical scavenging assay. The cytotoxicity of D. morbifera extract was analyzed through MTT assay using human normal oral keratinocytes. Results : D. morbifera extract showed antimicrobial activity against S. mutans and especially C. albicans. The solvent fractions of D. morbifera showed strong antimicrobial activity against S. mutans and C. albicans in n-hexane solvent fraction and butanol solvent fraction, respectively. The composites containing D. morbifera extract appeared to have larger antimicrobial activity against S. mutans and C. albicans compared to the composites without D. morbifera extract. D. morbifera extract also showed outstanding antioxidant activity. Among solvent fractions of D. morbifera, butanol, ethyl acetate, and chloroform solvent fraction tended to have increased antioxidant activity as the concentration increased. There was almost no antioxidant activity of the composites using D. morbifera extract. D. morbifera extract showed high cell survival rate in cytotoxicity test. Conclusions : As a result of this preceding study, D. morbifera extract turned out to have antimicrobial, antioxidant activity and cytophilicity. Based on these results, it is expected that D. morbifera is applicable as a ingredient for natural mouthwash and denture cleanser