한국인 구강에서 분리된 세균의 동정

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The aim of this study was to identify bacteria isolated from a Korean oral cavity and to determine their antimicrobial susceptibility against eight antibiotics. The bacterial strains were obtained from the Korean Collection for Oral Microbiology (KCOM). The bacteria were identified by comparing 16S rDNA sequences at the species level. The data showed that 77 bacterial strains were predominantly identified as streptococci (49.4%) and staphylococci(14.3%). Among the Mitis group streptococci, the 16S rDNA homogeneity was too high to discriminate between clinical strains at the species level. So, phylogenetic analyses of the Mitis group streptococci were evaluated using the partial gene sequence comparison of 16S rDNA, rpoB, sodA, and groEL. Phylogenetic data based on the nucleotide sequences of the four genes showed that Mitis group streptococci were not discriminated at the species level. Minimum inhibitory concentrations (MIC) were determined using a broth dilution assay to test the sensitivity of the bacteria isolated from a Korean oral cavity against eight antibiotics. The MIC values of the oral bacterial strains against antibiotics were different. Streptococci showed sensitivity toward clindamycin and vancomycin and were resistant to tetracycline. Staphylococci also showed sensitivity toward clindamycin, cefuroxime axetil and vancomycin and were resistant to penicillin antibiotics. Gram-negative bacterial strains showed sensitivity toward tetracycline and were resistant to clindamycin. The resulting data regarding the antimicrobial susceptibility test are useful in the decision for antibiotic treatments and needed to prevent the misuse or abuse of antibiotics.
Alternative Title
Identification of the bacteria isolated from oral cavities in a Korean population
Alternative Author(s)
Choi, Mi-Hwa
일반대학원 치의생명공학과
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Table Of Contents
I. 서 론 1

II. 연구재료 및 방법 4
1. 세균 및 세균배양 4
2. 세균 genomic DNA의 추출 4
3. Multilocus sequence typing (MLST) 5
4. PCR 증폭물의 클로닝 및 플라스미드 DNA 추출 5
5. 핵산염기서열 결정 및 상동성 검색 6
6. 계통발생 분석(Phylogenetic analysis) 6
7. 항생제 감수성 실험 6

Ⅲ. 연구 결과 12
1. 구강 병소에서 분리된 세균 동정 12
2. Mitis 군 연쇄구균의 계통발생 분석 13
3. 구강세균들의 수종 항생제에 대한 최소성장억제농도 14

Ⅳ. 총괄 및 고안 24

Ⅴ. 결 론 28

Ⅵ. 참 고 문 헌 29
조선대학교 대학원
최미화. (2014). 한국인 구강에서 분리된 세균의 동정.
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General Graduate School > 4. Theses(Ph.D)
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