시판 에센셜오일의 성분 분석 및 생리활성 효과

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Chemical Composition and Biological Activity of Commercially Available Essential Oils

Yu, Yeong Wol
Advisor : Prof. Lee, Mi Ja
Department of Complementary and Alternative Medicine, Graduate School of Chosun University

The essential oils, well-known aroma compounds, have extensively been used in health care or pharmacology field. This study was studied the chemical compositions of five kinds of commercially available essential oils in Korea and their antimicrobial and antioxidant activities. The chemical compositions of oils were analysed by gas chromatography and mass-spectrometry (GC-MS), their antimicrobial activities against forty bacterial strains by tube dilution method, antioxidant activities by 1,1-diphenol-2-picrylhydrazyl (DPPH) assay and the measurement of reducing power, and the further elucidation of cell protective effect of essential oils by lactate dehydrogenase (LDH) assay.
The minimum inhibitory concentrations (MIC) were in the ranges of 0.0625-0.5% (v/v) for lemongrass; 0.0625-1.0% for manuka; 0.025-1.0% for tea tree; 0.0625-1.0% for thyme and 1-4% for ravensara. This study showed that, among the essential oils tested, lemongrass and thyme oil had broad antimicrobial spectrum against infectious bacteria. Antioxidant activities of the essential oils were determined by DPPH and reducing power. Thyme oil showed the strongest antioxidant activity among 5 oils, compared with ascorbic acid. Thyme pre-treated SH-SY5Y cells showed slightly decreased release of LDH in the concentration of 5 ㎍/㎖ solely among five oils. Thyme also attenuated somewhat rotenone-induced intra-cellular reactive oxygen species (ROS) production in cells in a concentration-dependent manner.
This study will be the report on the chemical composition of commercially available essential oils in Korea and on antimicrobial activities against gram positive or negative standard strains and multi-drug resistant (MDR) bacterial strains isolated in domestic area.

Key Words: Essential oil, Antioxidant, Antimicrobial, Reactive oxygen species
Alternative Title
Chemical Composition and Biological Activity of Commercially Available Essential Oils
Alternative Author(s)
Yu Yeong Wol
일반대학원 보완대체의학과
Awarded Date
Table Of Contents
목 차

표 목차 ii
도 목차 iii
I. 서 론 3
II. 연구재료 및 방법 5
A. 재료 및 시약 5
B. 균주 5
C. 성분분석(GC, GC-MS) 6
D. 항미생물 활성측정 6
E. DPPH 라디칼 제거 효능 검색 7
F. 환원력 측정 7
G. 세포주 배양 8
H. MTT assay 8
I. Lactate dehydrogenase (LDH) 측정 8
J. 세포내 활성산소(Reactive oxgen species, ROS) 측정 9
III. 결과 10
A. 에센셜오일의 구성 성분 분석 10
B. 에센셜오일의 항미생물 활성 평가 16
C. 에센셜오일의 항산화 효능 평가 28
D. 에센셜오일에 의한 세포 생존율 변화 31
E. 에센셜오일의 세포 손상 억제 평가 33
F. 에센셜오일의 세포내 활성산소 생성 억제 효과 평가 35
Ⅳ. 고 찰 37
Ⅴ. 결 론 39
참 고 문 헌 40
조선대학교 대학원
유영월. (2014). 시판 에센셜오일의 성분 분석 및 생리활성 효과.
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General Graduate School > 4. Theses(Ph.D)
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